Labelling

Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells.

Propidium iodide (PI) is an ethidium bromide analog that emits red fluorescence upon intercalation with double-stranded DNA. PI does not permeate viable cell membranes, but passes through disturbed cell membranes and stains the nuclei. PI is often used in combination with a fluorescein compound, such as Calcein-AM or FDA, for simultaneous staining of viable and dead cells.

*Applicable to live and fixed cells *High retentivity of reagents with low toxicity *Just add reagents into medium

*Applicable to live and fixed cells *High retentivity of reagents with low toxicity *Just add reagents into medium

Rhod 2 has the longest fluorescent wavelength signal of all the calcium indicators. It has a rhodamine-like fluorophore whose excitation and emission maxima are 557 nm and 581 nm, respectively. This makes it a convenient excitation source for argon and krypton lasers. Although it was thought that the fluorescent signal of Rhod 2 temp-only increases several times with the calcium complex, Dojindo’s Rhod 2 increases its signal with calcium 8-1 times because of its high purity. Its signal intensity is the strongest of all the calcium probes.

MitoRed is a cell-membrane-permeable, rhodamine-based dye. It localizes in mitochondria and emits red fluorescence. The interaction of MitoRed with mitochondria depends on the membrane potential of the mitochondria.

SBD-F is a water-soluble reagent that reacts with sulfhydryl groups to produce highly fluorescent compounds. 　Application: Thiol compound derivatization for HPLC analysis

SBD-F is a water-soluble reagent that reacts with sulfhydryl groups to produce highly fluorescent compounds. 　Application: Thiol compound derivatization for HPLC analysis

SSP4 (Sulfane Sulfur Probe 4) is a novel fluorescent probe to detect sulfane sulfurs selectively. SSP4 itself is non-fluorescent, but it emits strong green fluorescence when it reacts with sulfane sulfurs. Thus, SSP4 enables high sensitive fluorescence detection and imaging of sulfane sulfurs.