Labelling

2,3,4-(PEG4-Azide)-1-(PEG4-Amine)methane is a four-armed PEG reagent that combines three azide groups with a primary amine. Azides are frequently used in click reactions with terminal alkynes, DBCO and BCN moieties to form stable triazole linkages with the target molecule. Primary amines are useful scaffolds for easily building complexity such as in making amides, building heterocycles, and performing SNAr reactions on electron-deficient aromatic compounds. PEG linkers increase a molecule’s water solubility and their use impacts a compound’s ADME properties in vivo.

Fluorescein-PEG12-Mal is a PEG linker containing a maleimide and a fluorescein dye. Maleimide is a thiol-specific covalent linker which is used to label cysteine residues in proteins while fluorescein is a fluorophore is a near-UV/Vis spectrum dye that can be used for in vivo fluorescence assays such as FRET.

BP Lipid 319 is a multi-ionizable amino lipid analogue of ALC-0315 featuring a propylamino headgroup and two acyl tails, one being a branched symmetrical C17 and the other being an asymmnetrical C11 chain. Ionizable lipids such as this may be applied in the development of lipid nanoparticles. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

BP Fluor 555 active ester is a bright, photostable, and hydrophilic fluorescent dye for the yellow channel. This NHS ester is an amine-reactive dye; it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules. BP Fluor 555 is a hydrophilic fluorophore with high fluorescence quantum yield and high photostability, an alternative to tetramethylrhodamine (TAMRA, TMR) or Cyanine3 dyes. The dye is useful for many applications, including cytometry and microscopy.

BDP TMR is a borondipyrromethene dye for the TAMRA channel, analog of BODIPY TMR. The dye has much brighter fluorescence than TAMRA. Because of the rather long lifetime of the excited state, this fluorophore is a good choice for fluorescence anisotropy measurements. This derivative is an amine-reactive NHS ester with an aminohexanoic acid (C6) linker between the dye and the functional group. The dye is suitable for labeling proteins, peptides, and other molecules with amino groups.

BDPep FL is a bright and photostable fluorophore for the fluorescein (FAM) channel. BDPep FL is an acid-stable version of BDP FL, keeping similar spectral properties to its ancestor. The compatibility of BDPep FL with TFA with additives used in solid phase peptide synthesis (SPPS) is a crucial advantage of this dye compared to other analogs. BDPep FL is beneficial for peptide synthesis due to its high stability and the absence of the dye decomposition to the corresponding dipyrrin under acidic conditions.

DusQ 1 NHS ester derivative is used for labeling with the primary amines of biomolecules. DusQ 1 is a dark quencher with an absorbance maximum of 522 nm, it can be used in qPCR probes applications, and multiplex assays. In FRET applications as an acceptor pairing with fluorophores, that emit in green to yellow range including FAM, JOE, VIC, R6G, HEX, TET.

DusQ 2 succinimidyl ester is a derivative with an amine reactive group for labeling with the amine-modified oligonucleotides, amine-containing proteins and other molecules. DusQ 2 is a nonfluorescent quencher with an absorbance maximum of 552 nm. It is useful as an acceptor in fluorescence resonance energy transfer (FRET) applications, nucleic acid assays in conjunction with yellow and orange to red emitting dyes such as Cyanine3, Cyanine5, ROX, Texas Red, AF 555, AF 594, AF 647. NHS ester function allows to conjugate the quencher to various biomolecules of interest, and also to prepare oligonucleotides with DusQ 2.

DusQ 21 is a dark quencher used as an acceptor in FRET applications, it has absorption maximum 656 nm, and can be used in multiplex assays due to the lack of secondary fluorescence. It is a quencher of choice for pairing with fluorescent dyes that emit in the red and near-infrared part of the range. DusQ 21 NHS ester derivative is used for labeling with the primary amines of amine-containing molecules. It can be used for quenching of many fluorophores, including Cyanine5, Cyanine5.5, AF 647.