Results for Labelling ( 3073 )
BDP 576/589, a boron dipyrromethene dye, is an analog of BODIPY™ 576/589 dye. This hydrophobic dye with emission in the orange spectrum range has high quantum yield and high molar extinction coefficient. It is suitable for experiments measuring fluorescence lifetime and fluorescence polarization. This fluorophore can be used whether in wide-field and confocal microscopy or in multiphoton microscopy because of its high two-photon absorption cross-section. This derivative is a carboxylic acid (contains a free COOH-group). It can be used as a reference control and for conjugation with an amino group after activation with carbodiimides.
DusQ 21 is a fluorescence quencher. DusQ 21 possesses broad absorption in far red and NIR range, and can quench fluorescence of dyes that emit in this region. This is a carboxylic acid derivative. DusQ 21 has intense absorption maximum at 661 nm, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications. It is a common quencher for Cyanine5, Cyanine5.5, AF 647, or other spectrally similar fluorescent dyes.
BP Fluor 488 is a stable dye over a broad pH range (from 4 to 10) with the fluorescence peak in the green region (at 519 nm). Conjugates of biomolecules with BP Fluor 488 can be used in flow cytometry, for example in multicolor staining. BP Fluor 488 is also used in cell microscopy due to its high photostability. BP Fluor 488 amine is soluble in water and can be conjugated with electrophiles and participate in enzymatic transamination.
This product is an amine derivative of ROX (Rhodamine X, Rhodamine 101) dye with an amine group separated from the fluorophore by a long linker that facilitates conjugation. The aliphatic primary amine group can be coupled with various electrophiles (activated esters, epoxides, etc.) and used in enzymatic transamination labeling. ROX is a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This reagent is a pure 6-isomer.
ROX tetrazine is a derivative of ROX (Rhodamine X, Rhodamine 101) dye, a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This compound contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick and specific. This reagent is a pure 5-isomer of ROX.
Tetrazine - trans-cyclooctene ligation is among the fastest bioconjugation reactions known to date. This water soluble sulfo-Cyanine3 derivative contains methyltetrazine moiety for the coupling with trans-cyclooctenes. Methyltetrazines possess optimal stability at physiological pH, while maintaining extremely high reactivity towards cyclooctenes. The fluorophore is bright, photostable, and is also very well visible with naked eye.
TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefins. sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation-based labeling. This reagent possesses good aqueous solubility and stability in biological environments.
sulfo-Cyanine5.5 is a water-soluble, hydrophilic far-red fluorophore, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore and minimize non-specific binding. This reagent contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes to form stable conjugates via an inverted electron demand [4+2]-cycloaddition (IEDDA). The reagent possesses high aqueous solubility and hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.