Labelling

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines. BP Fluor 568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm. BP Fluor 568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.

Strain promoted alkyne azide cycloaddition (SPAAC) between strained cycloalkynes and azides is a useful bioconjugation method that eliminates the need for copper catalyst necessary for classical CuAAC click chemistry. Cyanine5.5 DBCO is a cyclooctyne product containing Cyanine5.5 fluorescent dye. This fluorophore is used in bioimaging, biodistribution studies, and microscopy. Cyanine5.5 DBCO can be used to conjugate the fluorophore with various azides.

This product is a derivative of tetramethylrhodamine (TMR, TAMRA) containing a cyclooctyne moiety (dibenzocyclooctyne, DBCO). Pure 5-isomer. DBCO reacts quickly and efficiently with azides by simply mixing the components without the need for a copper catalyst (so-called sterically promoted cycloaddition reaction (SPAAC)). TAMRA DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. TAMRA is often used as a FRET acceptor for FAM fluorophore. Can replace DyLight 549.

sulfo-Cyanine3 DBCO is a water-soluble fluorescent dye with cycloalkyne group for copper-free click chemistry. DBCO (dibenzocyclooctyne) is a cyclooctyne with very high reactivity towards azides, still possessing good stability.

Copper-free click chemistry reaction between strained cycloalkynes (cyclooctynes), and azides, is a very fast and robust reaction. It can be used for a fast labeling with fluorescent dyes. This reagent is a derivative of water soluble sulfo-Cyanine5 dye which emits in red channel. It is useful for the labeling of biomolecules in aqueous media.

Cyanine3.5 dimethyl is a non-sulfonated cyanine dye with good solubility in organic solvents and bright fluorescence in the yellow-orange spectrum range. The dye can be used as a non-reactive fluorophore for control experiments, calibration, and other technical applications.

Non-functionalized Cyanine7.5 dye derivative with an aliphatic side chain and good solubility in organic solvents. The dye can be used as a non-reactive fluorophore for control experiments and for calibration.

Indocyanine Green (aka ICG, IC Green, Foxgreen) is used for reliable imaging and flow cytometry. This dye is water-soluble and pH-insensitive. The fluorescence of Indocyanine Green is not visible to the human eye but is readily detected by most imaging systems. Indocyanine Green binds tightly to plasma proteins and becomes confined to the vascular system. The dye has a half-life of 150 to 180 sec and is removed from circulation exclusively by the liver to bile juice. Indocyanine Green is used for determining cardiac output, hepatic function, liver and gastric blood flow, and for ophthalmic angiography.

TechnoDye 498/505 is a water soluble fluorophore. Its absorption and emission spectra are similar to fluorescein, but it is fluorescent in acidic media, and it is much more stable against photobleaching and oxidation.