Labelling

ChromaLINK Biotin Maleimide, 10 mg, contains a UV-traceable chromophore, enabling reproducibility in the biotinylation process. Measure degree of biotinylation without the standard curves needed for HABA/avidin and fluoro-reporter assays. With a simple UV scan, quantify biotin incorporation and ensure reproducible production of batches. The maleimide group reacts with free thiol groups on biomolecules and surfaces, such as the hinge region of antibodies after mild reduction of sulfhydryl groups.

AO is utilized for the detection of both double-stranded DNA and single-stranded DNA or RNA.

Annexin V specifically binds to phosphatidylserine in the presence of protein-dependent Ca ion. By using fluorescent-labeled Annexin V, the apoptotic cells can be identified by flow cytometry or fluorescence microscopy.

Bathocuproinedisulfonic acid is a water-soluble analog of bathocuproine that emits fluorescence under UV light.

BCECF is the most widely used intracellular pH probe. BCECF is highly water-soluble because it has 4 to 5 negative charges at neutral pH; it becomes difficult to pass through the cell membrane after loading. Its pKa value, 6.97, is higher than that of carboxyfluorescein. BCECF has an isosbestic point at 439 nm in the excitation spectra, so it can be used in ratiometry, similar to Fura 2.

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent

Biotin labeling reagent