Labelling

Calcein is a fluorescent metal indicator for calcium detection with EDTA titration. 

Calcein is a fluorescent metal indicator for calcium detection with EDTA titration. 

Calcein Blue solution emits bright blue fluorescence (λem=445 nm) at pH 4-11, but no fluorescence at pH 12 or higher. The blue fluorescence of the Calcein Blue solution at pH 4-11 is quenched by metal ions such as Co, Cu, Mn, Ni, and Pb. However, Calcein Blue forms a highly fluorescent complex with alkaline earth metals at pH 12 or higher. Since the endpoint of the chelate titration is clear by UV irradiation at 2-37 nm in a dark room, highly stained samples can be used. Calcein Blue is also suitable to determine Fe and Zr ions. Calcein Blue-Zn chelate can detect 1 ppb of F-. Calcein Blue aqueous solution emits laser at 449-49 nm.

CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy.

Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity.

CFSE is utilized for detection of viable cell as well as for the long-term observation of cell activities by fluorescent microscopy.

Calcein-AM is the most suitable fluorescent probe for staining viable cells because of its low cytotoxicity.

CytoRed is cell membrane permeable and accumulates inside of viable cells as resorufin. CytoRed has a much wider spectrum than BCECF or Calcein, so filters for fluorescein and rhodamine can also be used.

- Selective staining possible - Fast results - Fluorescent microscopy and flow cytometry detection CTC can be used in various ways to detect bacteria, depending on the objective.