Buffers & Concentrates

Tris-Acetate-EDTA (TAE) is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TAE suitable for multiple molecular biology applications including agarose electrophoresis for the separation of nucleic acids such as DNA and RNA.

TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. Tris-EDTA buffer is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.

Ammonium Acetate can be used for the precipitations of proteins and as a multipurpose buffer solution. Diethylpyrocarbonate (DEPC) is used to inactivate RNase enzymes in water and can also be used to affect the double-stranded structure of a DNA molecule. 5.0 M Ammonium Acetate is ideal for investigators in Molecular Biology, Cell Biology, and Immunology research.

5.0 M Potassium Acetate (untitrated) DEPC TREATED - In molecular biology, potassium acetate is used to precipitate dodecyl sulfate (DS) and DS-bound proteins, allowing the removal of proteins from DNA. It is also used as a salt for the ethanol precipitation of DNA. Potassium acetate is used in mixtures applied for tissue preservation, fixation, and mummification. Potassium acetate is used as a catalyst in the production of polyurethanes.

5.0 M Potassium Acetate (untitrated) DEPC TREATED - In molecular biology, potassium acetate is used to precipitate dodecyl sulfate (DS) and DS-bound proteins, allowing the removal of proteins from DNA. It is also used as a salt for the ethanol precipitation of DNA. Potassium acetate is used in mixtures applied for tissue preservation, fixation, and mummification. Potassium acetate is used as a catalyst in the production of polyurethanes.

GET buffer is used in effective plasmid miniprep.

Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

Saline–Sodium Phosphate–EDTA (SSPE) is used in bacterial screening and hybridization procedures.

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where species of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Northern blotting, in situ hybridization, or DNA microarrays). SSC is used to help control the stringency of hybridization in these steps.