Buffers & Concentrates

The Wash Buffer SSC is intended to be used for washing steps in <i>In Situ</i> Hybridization (ISH) on formalin-fixed, paraffin-embedded specimens.

The Wash Buffer A (25x) is intended to be used for washing steps in FISH on formalin-fixed, paraffin-embedded specimens.

The NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is suitable for extraction of cytoplasmic proteins in mild, non-denaturing conditions. 

The Modified NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is stronger than the standard NP-40 Cell Lysis Buffer. 

Please note this product may be subject to fees, we invite you to contact your local office. The Modified RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables the extraction of cytoplasmic and nuclear proteins in mild denaturing conditions. This is an SDS-free buffer optimized and formulated for proteins that might be sensitive to some components of the standard RIPA Buffer. This buffer is compatible with many applications, including ELISA-based assays.

Please note this product may be subject to fees, we invite you to contact your local office. The RIPA Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer enables extraction of cytoplasmic, membrane and nuclear proteins under denaturing conditions.

Please note this product may be subject to fees, we invite you to contact your local office. This kit contains four cell lysis buffers with varying detergent strengths (from mild to strong). This kit includes 20 ml of NP-40 Cell Lysis Buffer, Modified NP-40 Cell Lysis Buffer, Modified RIPA Lysis Buffer and RIPA Lysis Buffer. This kit is intended for optimization of mammalian cell lysis conditions that best suit the assay and protein of interest.

D-Luciferin K salt is a firefly luciferase substrate.

CUTANA™ Nuclei Extraction Buffer is the essential reagent for harvesting nuclei from cultured cells and tissues for use in CUT&RUN and CUT&Tag assays. This buffer is expected to be broadly compatible with eukaryotic cells and tissues. Below are examples of various cells and tissue types that have undergone successful nuclei isolation using this buffer: mouse NIH3T3 fibroblast cells human A549 non-small cell lung cancer (NSCLC) cells human K562 leukemia cells human NCI-H1299 non-small cell lung cancer (NSCLS cells) human bone marrow derived macrophages human TIG-1 fetal lung cells human monocyte derived macrophages human LoVo colorectal cancer cells human MV-4-11 macrophage cells human LNCaP prostate carcinoma cells human SUM149 triple negative breast cancer (TNBC) cells human renal primary cells human GM24385 B-lymphocyte (aka HG002) cells human peripheral blood mononuclear cells (PBMCs) human MCF7 breast cancer cells human intestinal tissue human MDA-MB-231 bre