Buffers & Concentrates

DMEM/F-12 Medium (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEM medium and Ham's F-12 medium, based on DMEM medium. F-12 was added to enrich the nutrient content, containing a variety of trace elements. Dulbecco Modified Eagle Medium (DMEM) is a modified Eagle Basic Medium (BME) with four times the amino acid and vitamin concentration of BME. Ham's F-12, based on Ham's F-10 medium, significantly increased the concentration of choline, inositol, putputylamine and several amino acids. DMEM/F-12 medium is widely used to support the growth of a variety of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the culture of mammalian cells with low serum content and clone density. DMEM/F12 Medium contains amino acids, vitamins, inorganic salts and other components required for

PEG Blocking Buffer for ELISA contain polyoxyethylene detergents that interfere with PEG and PEG antibody detection. PEGB125-1 effectively blocks and stabilizes PEG-protein and anti-PEG coated ELISA plates if used as directed.

PEG Dilution Buffer contains an effective blocking agent that minimizes non specific binding and a non polyoxyethylene detergent that decreases variability and background.

PEG Wash Buffer contains a non polyoxyethylene detergent that decreases variability and background.

Mammalian Cell Lysis Buffer (5X) requires minimal hands-on time and only needs a simple 5-fold dilution.

This product is a pre-cast gel for electrophoresis made with polyacrylamide. The Phos-tag contained in a gel during SDS-PAGE supplements the phosphoproteins, retarding the electrophoretic velocity of the phosphoproteins. In this way, the phosphoproteins and unphosphorylated proteins can be detected as separate bands.

 precast polyacrylamide gels containing Phos-tag for SDS-PAGE

This product is for research use only. Do not administer it to human.