Buffers & Concentrates

Tris HCL buffer

THE ES BUFFER IS AN ENDOTOXIN-SPECIFIC BUFFER THAT IS IDEAL FOR UNTREATED LAL. THOUGH ALL LAL REAGENTS PRODUCED BY FUJIFILM WAKO LAL ARE ENDOTOXIN-SPECIFIC, OTHER COMMERCIALLY AVAILABLE LAL REAGENTS ARE NOT. WITHOUT THIS BUFFER, TESTS CAN PRESENT A POTENTIAL FALSE POSITIVE WHEN TRYING TO DETERMINE THE ENDOTOXIN CONCENTRATION IN THE SAMPLE. THE ES BUFFER WORKS BY ADDING A LARGE AMOUNT OF CARBOXYMETHYLATED CURDLAN (CMC) TO LAL, THUS PREVENTING THE ACTIVATION OF LAL BY GLUCAN. THE PRESENCE OF LARGE AMOUNTS OF GLUCAN DOES NOT INTERFERE WITH THE QUANTITATION OF ENDOTOXIN. FUJIFILM WAKO FIRST MADE USE OF THESE FINDINGS BY DEVELOPING THIS ENDOTOXIN-SPECIFIC BUFFER, WHICH CONTAINS HIGH CONCENTRATIONS OF CMC. WHEN THE ES BUFFER IS USED TO RECONSTITUTE LAL, REAGENT BECOMES ENDOTOXIN-SPECIFIC. KEY FEATURES EACH VIAL HAS SOLUTION FOR USE IN RECONSTITUTING A MAX OF 5.2 ML OF REAGENT IDEAL FOR USE IN RECONSTITUTING ANY LAL THAT HAS THE POTENTIAL TO REACT WITH GLUCANS, THUS MAKING AN ENDOTOXIN-SPEC

WAKO PROUDLY PROVIDES HIGH-QUALITY, ENDOTOXIN FREE WATER IDEAL FOR ALL OF YOUR LAL TESTING NEEDS. PRODUCT FEATURES: • ENDOTOXIN-FREE WATER (<0.001 EU/ML) • STEAM STERILIZED BY USP STANDARDS • DERIVED FROM WATER FOR INJECTION, USP • NON LAL REACTIVE

WAKO PROUDLY PROVIDES HIGH-QUALITY, ENDOTOXIN FREE WATER IDEAL FOR ALL OF YOUR LAL TESTING NEEDS. PRODUCT FEATURES: • ENDOTOXIN-FREE WATER (<0.001 EU/ML) • STEAM STERILIZED BY USP STANDARDS • DERIVED FROM WATER FOR INJECTION, USP • NON LAL REACTIVE

The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics

The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics