Buffers & Concentrates

WAKO PROUDLY PROVIDES HIGH-QUALITY, ENDOTOXIN FREE WATER IDEAL FOR ALL OF YOUR LAL TESTING NEEDS. PRODUCT FEATURES: • ENDOTOXIN-FREE WATER (<0.001 EU/ML) • STEAM STERILIZED BY USP STANDARDS • DERIVED FROM WATER FOR INJECTION, USP • NON LAL REACTIVE

The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics

The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics

3 vials