Buffers & Concentrates

The 10X Whole Blood Lysing Solution is commonly used for lysing red blood cells following direct immunofluorescence staining of human peripheral blood cells with monoclonal antibodies prior to flow cytometric analysis.

DMEM/F-12 Medium (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a 1:1 mixture of DMEM medium and Ham's F-12 medium, based on DMEM medium. F-12 was added to enrich the nutrient content, containing a variety of trace elements. Dulbecco Modified Eagle Medium (DMEM) is a modified Eagle Basic Medium (BME) with four times the amino acid and vitamin concentration of BME. Ham's F-12, based on Ham's F-10 medium, significantly increased the concentration of choline, inositol, putputylamine and several amino acids. DMEM/F-12 medium is widely used to support the growth of a variety of mammalian cells, including MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium, and it is also suitable for the culture of mammalian cells with low serum content and clone density. DMEM/F12 Medium contains amino acids, vitamins, inorganic salts and other components required for

PEG Blocking Buffer for ELISA contain polyoxyethylene detergents that interfere with PEG and PEG antibody detection. PEGB125-1 effectively blocks and stabilizes PEG-protein and anti-PEG coated ELISA plates if used as directed.

PEG Dilution Buffer contains an effective blocking agent that minimizes non specific binding and a non polyoxyethylene detergent that decreases variability and background.

PEG Wash Buffer contains a non polyoxyethylene detergent that decreases variability and background.

Mammalian Cell Lysis Buffer (5X) requires minimal hands-on time and only needs a simple 5-fold dilution.

This product is a pre-cast gel for electrophoresis made with polyacrylamide. The Phos-tag contained in a gel during SDS-PAGE supplements the phosphoproteins, retarding the electrophoretic velocity of the phosphoproteins. In this way, the phosphoproteins and unphosphorylated proteins can be detected as separate bands.

 precast polyacrylamide gels containing Phos-tag for SDS-PAGE