Proteins & Peptides

Human PDE4B3 (GenBank Accession No. NM_001037340), 2-721 (end), with N-terminal GST tag, MW=108 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Human DNA polymerase γ (gamma), also known as Pol gamma and POLG (GenBank Accession No. NM_002693), a.a. 2-1239 (end), with N-terminal His-tag and N-terminal FLAG-tag, MW=141 kDa, expressed in a baculovirus infected Sf9 cell expression system.

Zaire Ebola Virus (Zebov) glycoprotein 1 (GP1), also known as envelope glycoprotein with a C-terminal His-tag, GenBank Accession No. NP_066246, MW=57.1 kDa, expressed in a HEK293 cell expression system. This protein runs at an apparent higher molecular weight due to glycosylation.

Zaire Ebola Virus (Zebov) glycoprotein 1,2 (GP1,2), also known as envelope glycoprotein with a C-terminal His-tag, a.a. 33-650, GenBank Accession No. NP_066246, MW=68.7 kDa, expressed in a HEK293 cell expression system. This protein runs at a higher apparent molecular weight due to glycosylation.

Zaire Ebola Virus (Zebov) glycoprotein 1,2 (GP1,2), also known as envelope glycoprotein, and GP, a.a. 33-650 with a C-terminal Avi and His-tag, GenBank Accession No. NP_066246, MW = 70.6 kDa, expressed in a HEK293 cell expression system. The protein was enzymatically biotinylated using AviTag™ technology. This protein runs at a higher apparent molecular weight due to glycosylation.

1,10-phenanthroline (o-phenanthroline) is a chelator with high affinity for divalent metal ions. When complexed with copper, it possesses nuclease activity that has been used to study DNA-protein interactions. While not specific for DUBs, it has been shown to be a potent inhibitor of JAMM-type isopeptidases through chelation of the active site Zn²⁺ ion. As such, o-phenanthroline is widely used when preparing cell lysates for enrichment of proteins bearing K63-linked polyubiquitin chains which are especially sensitive to DUBs in the JAMM family.

Human PRL 1 (GenBank Accession No. BC023975), a.a. 2-173 with N-terminal His-tag, MW = 19.8 kDa, expressed in an E. coli expression system.

Human PRL 2 (GenBank Accession No. NM_080391), a.a. 2-167 with N-terminal His-tag, MW = 19.1 kDa, expressed in an E. coli expression system.