Proteins & Peptides

Human GRK6, also known as GPRK6 or FLJ3213 (GenBank Accession No. BC_017272), full length, with N-terminal GST tag, MW ~93 kDa, expressed in Sf9 insect cells via a baculovirus expression system.

Complex of human LKB1, also known as STK11 (GenBank Accession No. NM_000455, a.a. 2-433, with N-terminal His-tag), MO25 (GenBank Accession No. NM_016289, a.a. 2-341, with N-terminal GST-tag), and STRAD (GenBank Accession No. NM_001003787, a.a. 2-431, with N-terminal GST-tag). Calculated MW=49 kDa* (LKB1), 66 kDa (MO25), and 74 kDa (STRAD), expressed in Sf9 cells using a Baculovirus expression system. *LKB1 runs anomalously on SDS-PAGE.

Recombinant full-length human p38alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST-tag. The gene accession number is NM_139012.

Mouse JNK1 (GenBank Accession No. NM_016700), full length with N-terminal GST-tag, MW=71 kDa, expressed in a Baculovirus infected Sf9 cell expression system. The mouse amino acid sequence of JNK1 is identical to the one of human JNK1.

Human PAK1 (GenBank Accession No. NM_002576.4), a.a. 248-545, MW=33.6 kDa, expressed in E. coli. PAK1 is fully phosphorylated at T423 and partially phosphorylated at additional sites.

Human PAK2 (GenBank Accession No. NM_002577), a.a. 227-524, MW=33.5 kDa, expressed in E. coli. PAK2 is fully phosphorylated at T402 and partially phosphorylated at additional sites.

Human recombinant LOK1, also known as STK10 (GenBank Accession No. NM_005990), a.a. 1-348 with N-terminal His-tag, expressed in a Baculovirus infected Sf9 cell expression system. MW = 43.2 kDa.

Human MEK1 (K97R) (GenBank Accession No. NM_002755), full length with lysine97 to arginine mutation and C-terminal His-tag, MW=44.3 kDa, expressed in E. coli. The K97R mutation prevents ATP binding, making the kinase inactive.

Human PAK4 (GenBank Accession No. BC002921), a.a. 300-591, MW=33.3 kDa, expressed in E. coli. PAK4 is fully phosphorylated at S474.