Lab Tools

The Transcription Factor Fixation/Permeabilization solution can be used with the relevant antibodies to stain for transcription factors such as Foxp3 and intranuclear staining of cytokines, chemokines, and nuclear proteins. The concentrated solution should be used with the Transcription Factor Fixation/Permeabilization Diluent (cat#92555-00) and Permeabilization Buffer (cat# 92110-00).

BG iMatrix 511 is a chemically defined, animal-free, and xeno-free laminin-511 E8 fragment. Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface and acts as a substrate for stem cell culture media for various cell types including ESCs and iPSCs. Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin.</p> The 350ug size is supplied as 2x175ug vials. The 1050ug size is supplied as 6x175ug vials.    </p> <span style="font-size: small;">Review the <a title="Data Sheet" href="https://bio-gems.com/media/product_pdfs/ECMs/BG%20iMatrix-511.pdf" target="_blank" rel="noopener">Data Sheet</a> and <a title="Brochure" href="https://bio-gems.com/media/product_pdfs/ECMs/ECM_Marketing_Imatrix%20511_Imatrix%20511%20Silk_Brochure.pdf" target="_blank" rel="noopener">Product Brochure

BG iMatrix 511 Silk is a chemically defined, animal-free, and xeno-free laminin-511 E8 fragment. Laminin-511 is well known to bind to integrin α6ß1, which is located on the cell surface and acts as a substrate for stem cell culture media for various cell types including ESCs and iPSCs. Recombinant laminin-511 E8 fragments are truncated, functional forms of laminin composed of the C-terminal regions of the alpha, beta, and gamma chains. Laminin-511 E8 (150 kDa) retains the full capability for binding to alpha 6 beta 1 integrin. The 350ug size is supplied as 2x175ug vials. The 1050ug size is supplied as 6x175ug vials.   <span style="font-size: small;">Review the <a title="Data Sheet" href="https://bio-gems.com/media/product_pdfs/ECMs/BG%20iMatrix-511%20Silk.pdf" target="_blank" rel="noopener">Data Sheet</a> and <a title="Brochure" href="https://bio-gems.com/media/product_pdfs/ECMs/ECM_Marketing_Imatrix%20511_Imatrix%20511%20Silk_Brochure.pdf" target="_blank" rel="noopener">Product Brochu

The ABTS (2,2?-azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) substrate is supplied as a one component, ready-to-use solution for ELISA applications. A soluble blue-green reaction product is obtained when this substrate system is reacted with horseradish peroxidase.

The ABTS (2,2?-azino-bis [3-ethylbenzthiazoline-6-sulfonic acid]) substrate is supplied as a one component, ready-to-use solution for ELISA applications. A soluble blue-green reaction product is obtained when this substrate system is reacted with horseradish peroxidase.

BioFX? Alkaline Phosphatase/Horseradish Peroxidase Block is supplied as a ready-to-use blocking reagent for immunohistochemistry samples. The solution blocks both alkaline phosphatase and peroxidase endogenous enzymatic activity.

Absorbance is read in the yellow spectral range of 405 nm - 420 nm.

Absorbance is read in the yellow spectral range of 405 nm - 420 nm.

Chemiluminescent substrates provide a higher degree of sensitivity than other substrates to enable lower limits of detection to be achieved in ELISA and membrane assays. The increase in sensitivity requires more vigilance during the optimization process through the proper selection of the dioxetane substrate, antibody/antigen titers and blockers. Surmodics provides multiple 1-component AP chemiluminescent substrates with various intensities and wavelength emissions to allow the assay developer to choose the appropropriate substrate for the application. Features: Absorbance at 450 nm. High signal-to-noise ratios.