Lab Tools

DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5·2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be

This buffer is intended for use with mammalian cells during magnetic cell isolation or single cell processing. This buffer is optimized and formulated to reduce nonspecific binding and cell clumping

5X Assay buffer specially formulated for reducing non-specific binding and maximizing signal-to-background in TR-FRET Biomarker Assay Kits

5X Assay buffer specially formulated for reducing non-specific binding and maximizing signal-to-background in TR-FRET Biomarker Assay Kits

5X Assay buffer specially formulated for reducing non-specific binding and maximizing signal-to-background in TR-FRET Biomarker Assay Kits