Results for Lab Tools ( 1724 )
Preclinical and clinical successes of Adeno-associated virus (AAV)-mediated gene therapy have established AAV as an ideal and safe therapeutic vector. Moreover, these successes have motivated research in both discovering and engineering novel AAV capsids that are more selective and clinically desirable than existing capsids. When injecting AAV particles into animals, it is necessary to use highly purified particles that do not contain any residual AAV host cells. Isolating AAV particles from the AAV host cells is conventionally conducted using freeze-thaw or sonication methods. However, these methods are time consuming and carry significant amounts of proteins from the host cells. The AAV ONE-Extract™ Solution is a reagent for quickly extracting AAV particles from AAV host cells. This reagent provides a simple and efficient method for isolating the AAV particles and is suitable for all AAV serotypes. The resulting viral particles are well-suited for cell infection or further purifica
DAPI (4',6-diamidino-2-phenylindole) is a fluorescent dye which can bind DNA strands robustly, the fluorescence being detected by fluorescence microscope. DAPI can dye both live and fixed cells as it can cross intact membrane, with higher efficiency in fixed cells. The molecular formula is C16H15N5·2HCl with 350.25 g/mol molecular weight, CAS Number 28718-90-3. DAPI could pass through the cell and nucleic membranes and bind the double-strand DNA in the nucleus, producing 20 times stronger fluorescence than itself. The efficiency detected by fluorescence microscope is very high (almost 100%), having no side effects for the live cells. The sensitivity for double stranded DNA DAPI staining is many times larger comparing to ethidium bromide (EB). DAPI staining is usually used in cell death detection, as it enters more effectively and generates stronger fluorescence in dead cells. After staining with DAPI, detect with fluorescence microscope or flow cytometry. Blue fluorescent cell would be