Lab Tools

The 200x Ubi-Mix™ is a mix of Europium cryptate-labeled Ubiquitin (donor), Cy5-labeled Ubiquitin (acceptor), and unlabeled Ubiquitin at a ratio optimized for TR-FRET based assays where long and/or branched polyubiquitin chains are formed. Under conditions where short ubiquitin chains or multi-monoubiquitylation on a substrate protein are expected, a different reagent might be required. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains, using 200x Ubi-Mix™ avoids time-consuming washing steps and allows for monitoring polyubiquitination real-time kinetics

Microarray Scanner Calibration Slide is designed for users to perform quantitative evaluations of their microarray scanners. It can be used for determining the dynamic range, detection limit, uniformity of microarray scanners, and detecting laser channel cross-talk and laser stability. Each Calibration Slide features two separate blocks of microarrays of of Cy3 and Cy5 fluorescent dyes in dilution series. Each block consists of 28 sets of two-fold dilutions of Cy3 or Cy5, coupled with 3 sets of blanks and one set of position markers. Each column contains 12 repeats of each sample.