Results for Lab Tools ( 2275 )
Rockland Immunochemicals produces the following blocking buffer reagents: BSA, BLOTTO, BLOTTO A, BLOTTO B and Blocking Buffer for Fluorescent Western Blotting (see related products below). These products are intended to block non-specific binding of proteins in various immunological assays. Typically, western blotting and ELISA methods call for the addition of a blocking agent prior to and during incubation with primary antibody.
BCIP/TNBT (5-Bromo-4-chloro-3'-indolyphosphate p-toluidine salt/ Tetrazolium nitro blue tetrazolium chloride) is utilized in combination as a chromogenic substrate for alkaline phosphatase (AP) in various applications, including WB and IHC. The reaction produces an insoluble dark purple/blue precipitate. This substrate is particularly useful for chromogenic detection in blotting and in situ hybridization assays due to its high sensitivity and resolution.
DAB Membrane Peroxidase Substrate (50x) is a benzidine derivative that is oxidized by hydrogen peroxide in the presence of hemoglobin, yielding a dark-brown color of solution. This oxidation can be utilized for tissue samples by first preparing them with hydrogen peroxide. DAB Membrane Peroxidase Substrate (50x) is ideal for researchers in Immunology and Microbiology research.
FemtoMax™ Super Sensitive Chemiluminescent HRP Substrate is an extremely sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP). FemtoMax™ is designed for both Western blotting and enzyme-linked immunosorbent assay (ELISA) use. FemtoMax™ easily allows for the detection of femtogram (10-15) amounts of antigen using photographic film or other imaging methods, including highly sensitive CCD cameras. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of detection reagents and re-probed. Use the same blotting conditions for FemtoMax™ as you would when using Amersham ECL Plus™ Substrate or Pierce SuperSignal® West Femto Substrate.