Lab Tools

NPP (p-Nitrophenyl phosphate) is a colorimetric substrate for alkaline phosphatase (AP) in ELISA, producing a yellow product that is measurable at 405 nm. It is chosen for its suitability in applications requiring a quantitative readout of enzyme activity.

NPP Buffer (1x Working Buffer) is required with the use of NPP (50X) ELISA Alkaline Phosphatase Substrate a chromogenic substrate utilized in immunosorbent assays. Phosphatase-conjugated detection antibodies can act upon the pNPP to convert it to a yellow para-nitrophenol. This yellow product can be measured on a spectrophotometer at 405nm.

A magnetic separation unit designed to hold, and separate magnetic particles to the side of the tubes, up to five 15 mL and three 50 mL tubes. The unit contains five bar magnets embedded in a plastic housing.

Enhanced Chemiluminescence (ECL) involves the enzymatic oxidation of luminol, a process that is catalyzed by peroxidase (HRP) enzyme. In the presence of HRP and a peroxide, luminol is oxidized, and emits light. The intensity of the emitted light is proportional to the amount of HRP-bound target, allowing for the quantitative detection of proteins.

Enhanced Chemiluminescence (ECL) involves the enzymatic oxidation of luminol, a process that is catalyzed by peroxidase (HRP) enzyme. In the presence of HRP and a peroxide, luminol is oxidized, and emits light. The intensity of the emitted light is proportional to the amount of HRP-bound target, allowing for the quantitative detection of proteins.

X-Gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) is a chromogenic substrate for the β-galactosidase enzyme, commonly used in molecular biology for blue-white screening of recombinant bacterial colonies and other reporter assays. When hydrolyzed by β-galactosidase, X-Gal produces an insoluble blue precipitate.

5x concentrated reaction buffer for use with SMARCA Remodeling Enzymes (EpiCypher 15-1014 and 15-1015). Optimized for use with SMARCA Remodeling Enzyme, produced in SF9 cells. SMARCA is an ATP-dependent chromatin remodeling complex that regulates nucleosome spacing.

10X CMF-HBSS Concentrate, 1 bottle, 500 ml. Sterile calcium- and magnesium-free hank's Balanced Salt Solution (CMF-HBSS). The solution is used for washing and perfusing the liver prior to the addition of the dissociating enzyme solution. Store at 2-8°C.