Results for Bioconjugation Kits ( 196 )
BroadPharm MagicLink™ Sulfo-NHS-LC-Biotin is an intermediate-length, water-soluble biotinylation reagent for labeling antibodies, proteins and other molecules that have primary amines. <br>Features: <ul style="list-style-type: disc"><li><strong>Amine-reactive:</strong> reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides</li><li><strong>Soluble charged:</strong> sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds</li><li><strong>Medium length spacer arm:</strong> (total length added to target) is 22.4 angstroms; provides excellent balance between adequate length (minimal steric hindrance for biotin binding) and modest mass</li><li><strong>Irreversible forms:</strong> permanent amide bonds; spacer arm cannot be cleaved</li><li><strong>Cell surface:</strong> labeling biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes</li><li><
The BroadPharm Biotin Quantitation kit enables a quick estimation of the mole-to-mole ratio of biotin to protein. To quantify biotin label incorporation, a solution containing the biotinylated protein is added to a mixture of HABA and avidin. Because of its higher affinity for avidin, biotin displaces HABA from its interaction with avidin and the absorption at 500 nm decreases proportionately. By this method, an unknown amount of biotin present in a solution can be evaluated in a single cuvette by measuring the absorbance of the HABA-avidin solution before and after addition of the biotin-containing sample. The change in absorbance relates to the amount of biotin in the sample.<br /> <br /> For your biotinylating needs. refer to BroadPharm kits Sulfo-NHS-LC-Biotin kit and reagent (BP-50058), Sulfo-NHS-Biotin (BP-50056), and NHS-PEG4-Biotin (BP-50054)
HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™ chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibo
HRP can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is an issue. <br /> <br /> BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™ chemistry and kit do not need TCEP, DTT, or catalysts at all!<br /> First, antibo
Alkaline phosphatase (AP) can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is a concern. <br /> <br /> BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™chemistry and kit do not need TCEP, DTT, or catalysts at al
Alkaline phosphatase (AP) can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi-functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amounts of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is a concern. <br /> BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™chemistry and kit do not need TCEP, DTT, or catalysts at all! <br /
Alkaline phosphatase (AP) can be conjugated to antibody/protein by thiol-maleimide and hydrazone coupling approaches. The thiol-maleimide approach is commonly performed with a bi- functional linker (such as SMCC). However, SMCC-modified protein is extremely unstable and often self-reactive since it often contains both amine and thiol groups that cause significant amount of homo-crosslinking. The thiol-maleimide linkage is exchangeable when other thiol groups are present. In the hydrazone coupling approach, the conjugation reaction is based on a reaction between hydrazine and aldehyde. This method is low yielding although a catalyst is used. The size of the molecule and inaccessibility of the functional group contribute to low yield. In addition, linkage stability is a concern. <br /> BroadPharm's MagicLink™ technology solves all the problems by combining PEG and proprietary crosslinking chemistry. MagicLink™chemistry and kit do not need TCEP, DTT, or catalysts at all!<br />
R-Phycoerythrin (PE, 250K), a fluorescent protein from the phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.<br /> MagicLink™ is based on instant reaction between functional groups MAGIC and LINK. By following the easy protocol provided in the kits, the end users can activate their antibody with MAGIC NHS to get MAGIC-antibody. It is then instantly reacted with LINK pre-activated PE (provided in the kits) to achieve PE-antibody conjugates.<br /> These kits are specifically formatted and optimized to conjugate 10µg, 50µg, 100µg, and 500µg of antibodies.</p> <ul> <li>Novelty: 3rd generation protein crosslink technology. No DTT, TCEP, catalyst, quencher, or ligands needed.</li> <li>Yield: conjugation efficiency around 95-100%; no antibo
R-Phycoerythrin (PE, 250K), a fluorescent protein from the phycobiliprotein family, is isolated from red algae. Its primary absorption peak is at 565 nm with secondary peaks at 496 and 545 nm. It is excited by laser lines from 488 to 561 nm, with absorbance maxima at 496, 546, and 565 nm and a fluorescence emission peak at 578 nm.<br /> MagicLink™ is based on instant reaction between functional groups MAGIC and LINK. By following the easy protocol provided in the kits, the end users can activate their antibody with MAGIC NHS to get MAGIC-antibody. It is then instantly reacted with LINK pre-activated PE (provided in the kits) to achieve PE-antibody conjugates.<br /> These kits are specifically formatted and optimized to conjugate 10µg, 50µg, 100µg, and 500µg of antibodies.</p> <ul> <li>Novelty: 3rd generation protein crosslink technology. No DTT, TCEP, catalyst, quencher, or ligands needed.</li> <li>Yield: conjugation efficiency around 95-100%; no antibo