Click Chemistry

BP Fluor 647 is a water-soluble, pH-insensitive from pH 4 to pH 10 bright, far-red fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Cy5 or BP Fluor 647 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum. BP Fluor 647 DBCO is a bright, far-red fluorescent, probe routinely used for imaging of azide-containing biomolecules without the need for copper catalyst. BP Fluor 647 DBCO reacts with azides via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. In application where the presence of copper is a concern BP Fluor 647 DBCO is an ideal alternative to copper requiring fluorescent alkynes. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

BP Fluor 647 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper. In addition, the use of picolyl azide instead of conventional azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling. In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired. Reagent grade, for research purpose. Please contact us for GMP-grade i

TAMRA Azide, isomer 5 (also known as Tetramethylrhodamine 5-Carboxamido-(6-Azidohexanyl)) is the orange-fluorescent probe that is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. It is predominantly used for detection of terminal alkyne-tagged biomolecules via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. For the detection of low abundance targets or where significant increase in signal intensity is desired please consider using next generation azides probes containing an internal copper-chelating motif. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

TAMRA Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site. The rate of CuAAC reaction depends on concentrations of all reagents, including copper, thus the raise of the effective copper concentration at the reaction site dramatically increase the rate of CuAAC reaction without the need to increase concentration of azide reagent and copper. This probe is compatible with various excitation sources including mercury arc, tungsten and xenon arc lamps, the 544 nm line of the Helium-Neon laser and the 532 nm green laser line. In addition, the use of TAMRA Picolyl Azide instead of conventional TAMRA Azide allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling. In summary, the introduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of

Bis-sulfone-PEG12-Acid is a reagent with a sulfone group and a terminal carboxylic acid. The terminal acid reacts with primary amines with the help of activators (EDC or HATU) to from stable amide bonds. The sulfone group can be conjugated with thiol groups of proteins. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Panobinostat (LBH589) is an HDAC (histone deactylase) inhibitor of research interest for multiple diseases such as multiple myeloma. Panobinostat was reported to inhibit several human multiple myeloma cell lines with and IC50 ≥ 20nM. Panobinostat was also reported to induce expression of DNA damage response genes and induce apoptosis in Ph- acute lymphoblastic leukemia cells. Panobinostat has potential applications in several different forms of cancer, in addition to spinal muscular atrophy and HIV therapy. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Biotin-PEG4-phosphorylamino-inosine is a PEG linker that has terminal a biotin group and a Inosine 5′-monophosphate moiety. Biotin has strong binding affinity with avidin. 5′-monophosphate moiety can be used as a substrate to study the distribution, specificity, and kinetics of inosine-5′-monophosphate dehydrogenase (IMPDH). Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

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BTTP is a newest generation, accelerating ligand for CuAAC that provides much greater rate enhancement compared to previous generation ligands (e.g. THPTA or TBTA). More importantly, it minimizes perturbations to the physiological state of the cells or organisms probed and allows for effective bioconjugation with suppressed cell cytotoxicity by further lowering copper loading in the catalyst formulation. Nature Communications. 5: 4981(2014) Chem Asian J., 6(10), 2796-802(2011) Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.