Click Chemistry

Bis-sulfone Amine is a reagent featuring two sulfone groups and a primary amine. The sulfone group is a covalent ligand which is used to bind thiols via a Michael addition, and this can be employed to label cysteine residues in proteins. The presence of two leaving groups on this molecule allows this linker to bind two thiols at a time. The amine group is free to perform a variety of reactions such as coupling with carboxylic acids, reductive amination with aldehydes or ketones, or SNAr with electron-deficient aromatic groups.

BP Fluor 532 Alkyne is a bright, yellow-fluorescent, alkyne-activated probe that reacts with azides via a copper-catalyzed click reaction (CuAAC). This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of BP Fluor 532 dyes are well suited to direct imaging of low-abundance targets. The absorption/emission spectra of BP Fluor 532 is a perfect match to spectra of many other fluorescent dyes based on sulfonated rhodamine core, including Alexa Fluor® 532 and CF®532 Dye. For application where the presence of copper is not acceptable, please consider our BP Fluor™ 532 DBCO probe for copper-less detection of azide-modified molecules.

Azido Myristic Acid (also provided as Click-iT® myristic acid, azide) can be used to identify and characterize post-translationally myristylated proteins with using a simple and robust two-step labeling and detection technique. Due to the very small size of the azide group and its complete absence in any natural molecules the presence of azide group does affect the biodistribution of azido palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

Azido Palmitic Acid (also sold as Click-iT® palmitic acid, azide) can be used to identify and characterize post-translationally palmitylated proteins with using a simple and robust two-step labeling and detection technique. Due to the very small size of the azide group and its complete absence in any natural molecules the presence of azide group does not affect the biodistribution of azido palmitic acid in comparison to the natural palmitic acid making it a suitable substrate for enzymes.

BP Fluor 568 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

BP Fluor 594 TCO reacts with tetrazines to produce a stable, covalent linkage, also referred to as the inverse-electron demand Diels-Alder cycloaddition reaction. This reaction is extremely fast (k > 800 M -1 s-1), selective, biocompatible, and does not require Cu-catalyst or elevated temperatures. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date.

A bright, green-fluorescent probe used for detection TCO-tagged biopolymers. BP Fluor 488 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must BP Fluor 488 Tetrazine probe would of great value.

A bright, red-fluorescent probe used for detection TCO-tagged biopolymers. BP Fluor 568 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must BP Fluor 568 Tetrazine probe would of great value.

A bright, red-fluorescent probe used for detection TCO-tagged biopolymers. BP Fluor 594 Tetrazine demonstrates exceptionally fast cycloaddition kinetics (up to 30 000 M-1 s-1) with trans-cyclooctenes (TCO) as the dienophile, the fastest kinetics ever reported for any bioorthogonal reaction. In applications such as in vivo cancer imaging or pre‐targeted cell labeling studies where rapid reaction kinetics is a must BP Fluor 594 Tetrazine probe would of great value.