Click Chemistry

Whilst biotin is a highly effective affinity label, fluorescent labels provide a more sensitive, quantitative, and convenient method for visualizing proteins. Trifunctional click chemistry probes that incorporate a ligation handle, a biotin and a fluorophore straightforwardly extended to combine the complementary benefits of both types of label. Dual-label TAMRA Biotin Alkyne probe can be readily incorporated into azide-tagged biomolecules through CuAAC ligation. For example, dual-labeled newly synthesized proteins, glycans, lipids, or DNA/RNA can be readily isolated by affinity purification and visualized by in-gel fluorescence.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst. Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper-containing catalyst. Alkyne cholesterol represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

This reagent is a modified lipid containing an omega-terminal alkyne. The terminal alkyne group can be used in a highly specific linking reaction with azide-containing reagents, known as ‘click chemistry’, in the presence of a copper (Cu)-containing catalyst. E-Cholesterol Alkyne (19-ethynylcholesterol) represents a versatile, sensitive, and easy-to-use tool for tracking cellular cholesterol metabolism and localization as it allows for manifold detection methods including mass spectrometry, and fluorescence microscopy.

MB 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. MB 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. MB 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable (Figure 1). The superior photostability and excellent brightness of MB 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

BP Fluor 660R is a bright and photostable far-red dye that emits fluorescence at about 685 nm in the borderline spectral region between far-red and near-IR. Although the absorption maximum is at around 665 nm, this dye can be sufficiently excited by the 633 or 635 nm laser. BP Fluor 660R dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is exceptionally photostable. The superior photostability and excellent brightness of BP Fluor 660R make the dye an ideal choice for confocal microscopy and other demanding applications.

BP Fluor 680R is a bright and photostable near IR dye that emits fluorescence at about 700 nm in the borderline spectral region between far-red and near-IR. BP Fluor 680R dye is water soluble and pH-insensitive from pH 4 to pH 10. BP Fluor 660R is a rhodamine-based dye, and like rhodamine dyes in general, it is very bright and exceptionally photostable. The superior photostability and excellent brightness of MB 680R makes this dye an ideal for confocal microscopy, single molecule imaging and other applications that demand both brightness and photostability.

Mono-Sulfone Acid is a reagent featuring a sulfone group and a benzoic acid. The sulfone group is a covalent ligand which is used to bind thiols via a Michael addition, and this can be employed to label cysteine residues in proteins. The carboxylic acid is free to do couplings with amines or alcohols to form amides or esters respectively.

Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”. Proteins conjugated to DBCO-Gly-Gly-Gly can be further modified with azide-containing molecules creating site-specific protein conjugates. Examples of creating protein conjugates using sortagging include site-specifically PEGylating proteins,1 site-specific protein-lipid conjugates,2 and constructing peptides and glycosylphosphatidylinositol chimeras.3 Sortase has also been used in peptide synthesis to cyclize peptides to create macrocyclic peptides, glycopeptides4 and protein−protein conjugates.5