Click Chemistry

JOE dye is a fluorophore similar to TET, but less hydrophobic. The dye is often used for the labeling of nucleic acids. JOE alkyne can be conjugated with azides using copper catalyzed Click chemistry reaction.

ROX alkyne is an alkyne derivative of the bright red-emitting dye ROX (Rhodamine X, Rhodamine 101). Due to its quantum yield approaching unity, ROX is a popular dye for qPCR and microscopy applications. It is used for fluorescent labeling of azide-containing biomolecules via copper-catalyzed click reaction. This product is a pure 6-isomer of ROX. ROX is prone to oxidation; therefore, its derivatives should preferably be stored in an inert atmosphere.

ROX tetrazine is a derivative of ROX (Rhodamine X, Rhodamine 101) dye, a red-emitting fluorophore possessing high brightness and fluorescence quantum yield. This compound contains tetrazine moiety that reacts with trans-cycloalkenes and other strained olefins in inverse electron demand Diels-Alder reaction (IEDDA). The reaction is very quick and specific. This reagent is a pure 5-isomer of ROX.

Tetrazine - trans-cyclooctene ligation is among the fastest bioconjugation reactions known to date. This water soluble sulfo-Cyanine3 derivative contains methyltetrazine moiety for the coupling with trans-cyclooctenes. Methyltetrazines possess optimal stability at physiological pH, while maintaining extremely high reactivity towards cyclooctenes. The fluorophore is bright, photostable, and is also very well visible with naked eye.

TCO (tetrazine – trans-cyclooctene) ligation is one of the fastest reactions used for bioconjugation. It is an inverse electron demand [4+2] cycloaddition that takes place between tetrazine and trans-cyclooctene or other strained olefins. sulfo-Cyanine5 tetrazine is a fluorophore derivative bearing a tetrazine group for the TCO-ligation-based labeling. This reagent possesses good aqueous solubility and stability in biological environments.

sulfo-Cyanine5.5 is a water-soluble, hydrophilic far-red fluorophore, an analog of Cy5.5®. Like other cyanine dyes, sulfo-Cyanine5.5 has an outstanding molar extinction coefficient, giving rise to its bright fluorescence. The molecule contains four sulfo groups that provide hydrophilicity and negative charge to the fluorophore and minimize non-specific binding. This reagent contains tetrazine moiety that reacts with trans-cyclooctenes, cyclopropenes, and some strained cyclooctynes to form stable conjugates via an inverted electron demand [4+2]-cycloaddition (IEDDA). The reagent possesses high aqueous solubility and hydrophilicity and is recommended for labeling biomolecules in an aqueous environment.

Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines. BP Fluor 568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm. BP Fluor 568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.

Strain promoted alkyne azide cycloaddition (SPAAC) between strained cycloalkynes and azides is a useful bioconjugation method that eliminates the need for copper catalyst necessary for classical CuAAC click chemistry. Cyanine5.5 DBCO is a cyclooctyne product containing Cyanine5.5 fluorescent dye. This fluorophore is used in bioimaging, biodistribution studies, and microscopy. Cyanine5.5 DBCO can be used to conjugate the fluorophore with various azides.

This product is a derivative of tetramethylrhodamine (TMR, TAMRA) containing a cyclooctyne moiety (dibenzocyclooctyne, DBCO). Pure 5-isomer. DBCO reacts quickly and efficiently with azides by simply mixing the components without the need for a copper catalyst (so-called sterically promoted cycloaddition reaction (SPAAC)). TAMRA DBCO can be used for the labeling of proteins, peptides, nucleic acids, and other molecules containing azide groups. TAMRA is often used as a FRET acceptor for FAM fluorophore. Can replace DyLight 549.