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<i>Taq</i> DNA Polymerase is a thermostable DNA Polymerase isolated from an <i>E. coli</i> strain that carries the <i>Taq</i> DNA polymerase gene. <i>Taq</i> DNA Polymerase is the most common polymerase used for PCR.<br><br><li><b>Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see <a href=

<i>Taq</i> DNA Polymerase is a thermostable DNA Polymerase isolated from an <i>E. coli</i> strain that carries the <i>Taq</i> DNA polymerase gene. <i>Taq</i> DNA Polymerase is the most common polymerase used for PCR.<br><br><b>Note: This product is supplied with 10X reaction buffer containing 15 mM magnesium chloride. dNTP (10 mM) mixture must be ordered separately (see <a href="/enzyme/D0056-dNTP_mixture.html">related products</a>).</b>

DNA Polymerase is a thermostable DNA polymerase isolated from an <i>E. coli</i> strain that carries the <i>Taq</i> DNA polymerase gene. <i>Taq</i> DNA polymerase is the most common polymerase used for PCR.

<i>Taq</i> DNA Polymerase is a thermostable DNA polymerase isolated from an <i>E. coli</i> strain that carries the <i>Taq</i> DNA polymerase gene. <i>Taq</i> DNA Polymerase is the most common polymerase used in PCR. In some cases, such as RAPD PCR, adding large volume of general <i>Taq</i> DNA polymerase (5 U/μl), which has a high concentration of glycerol in its storage buffer, will increase the glycerol concentration in the reaction mix, interfering with PCR performance. The use of concentrated <i>Taq</i> DNA Polymerase (25 U/μl ), with a far slimmer dose of glycerol, can prevent poor PCR efficiency. <br><br><b>Note:</b> Concentrated <i>Taq</i> DNA Polymerase (GenScript, E00012) is supplied with 10X reaction buffer containing 15 mM magnesium chloride. The <a href="https://www.genscript.com/enzyme/D0056-dNTP_mixture_10_mM_each.html">dNTP (10 mM) mix</a> may be ordered separately.

2X <i>Taq</i> Master Mix is a premixed 2X concentrated solution of <i>Taq</i> DNA Polymerase (GenScript, Cat. No. E00007), reaction buffer, MgCl ₂ and dNTPs. 2X <i>Taq</i> Master Mix contains all components for PCR, except DNA template and primers. The mixture is optimized for consistent and efficient routine PCR amplifications. It can amplify up to 8 kb fragment from lambda DNA. For a 50 µl reaction, simply add 25 µl of 2X <i>Taq</i> Master Mix to primers, DNA template and PCR-Qualified H₂O. <br/>

Green <i>Taq</i> DNA Polymerase is designed to increase the stability of the <i>Taq</i> enzyme for more convenient transport and applications. The polymerase (1000 U) is designed for 400 rxns if 2.5 U are used per 50 ul PCR reaction. It can be stable for six months if stored at 4°C or for up to one month without significant loss of activity at ambient temperature. Our technology can increase the stability of Green <i>Taq</i> DNA at higher temperature (72°C). The higher yield PCR product can be harvested in long PCR amplification. </ul> <br/> <b>Formulation</b><br/> <b>PCR Reaction Buffer (with Mg ²⁺ ):</b> 100 mM Tris-HCl, 15 mM MgCl ² , 500 mM KCl and 1% Triton X-100, pH 8.3 (4°C). <br/> <b>Enzyme Storage Buffer:</b> 20 mM Tris-HCl, 1 mM DTT,0.1 mM EDTA, 0.1 M KCl, 0.5% Nonidet P40 (v/v), 0.5% Tween 20 (v/v), 50% glycerol(v/v), pH 8.0 (4°C) <br/> <br/> <b>Applications</b> <br/> The applications of Green <i>Taq</i> DNA Polymerase include the following: <ul

Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. <br/>

Hot Start Taq DNA Polymerase is a recombinant, thermostable Taq DNA polymerase complexed with a thermolabile, neutralizing antibody that blocks the polymerase activity prior to the initial DNA denaturation step of PCR. When the temperature of the PCR reaction mix reaches 95°C during the initial DNA denaturing step of PCR cycling, activity of the Taq DNA polymerase is fully restored. <br/>

GenScript M-MuLV Reverse Transcriptase (M-MLV) is derived from a cloned region of the pol gene of MMLV and isolated from an E. coli strain overexpressing this construct. To increase cDNA yields and get a higher percentage of longer transcripts, the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity, and expressed free of exogenous RNases and other nucleases. The enzyme can synthesize a complementary cDNA strand initiating from a primer using RNA as template (cDNA synthesis), making it ideal for a wide range of applications.