DNA & Clones

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2, 50-N-66, Biotinylated is a 263 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see EpiCypher 18-0005), as well as both a 5’ and 3’ acceptor sequence to accommodate the histone octamer subsequent remodeling. ST601-GATC1,2, 50-N-66 DNA has restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.

EpiDyne® Remodeling Assay Substrate DNA ST601-GATC1,2,3, 50-N-66, Biotinylated is a 263 base-pair double-stranded DNA fragment containing a 5’ biotin-TEG group. This sequence includes the Lowary 601 nucleosome positioning sequence (see EpiCypher 18-0005), as well as both a 5’ and 3’ acceptor sequence to accommodate the histone octamer subsequent remodeling. ST601-GATC1,2,3, 50-N-66 DNA has restriction enzyme sites embedded in the 601 sequence that are accessible after nucleosome remodeling.

EpiDyne®-FRET Remodeling Assay Substrate DNA is a 207 base-pair double-stranded Cy3-conjugated DNA fragment. This sequence includes the Lowary 601 nucleosome positioning sequence (see 18-0005), a 5’ Cy3 fluorophore, as well as a 3’ acceptor sequence to accommodate the histone octamer subsequent to remodeling. The DNA also has a restriction enzyme site embedded in the 601 sequence that is accessible after nucleosome remodeling by the DpnII restriction enzyme.