Nucleotides

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.). In vitro studies showed that N6-methyl ATP substituted for ATP and supported cytoskeletal filament-driven translocation of motor proteins (dynein, kinesin and myosin) (Schliwa et al. & Shimizu et al.). N6-methyl ATP is also a substrate for RNA polymerase and can be used for preparation of modified RNA (Rohayem & Kariko et al.).

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dC and dU are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dCTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dC and dU are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dCTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dCTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dC and dU are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dCTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dU and dC are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dUTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dU and dC are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dUTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

2'-Fluoro NTPs are being utilized in an increasing number of applications in research and new drug development. 2'-Fluoro-dUTP is incorporated in both DNA and RNA constructs to improve in vivo stability. Both 2' Fluoro dU and dC are used in the design and synthesis of aptamers, antagomirs and siRNA, because they impart increased target affinity and resistance to endonuclease degradation, while reducing immune response. A key technique utilized in aptamer development using 2'-Fluoro-dUTP is SELEX (Systematic Evolution of Ligands by Exponential Enrichment). The SELEX process allows for the simultaneous screening of 1 x 1015 different oligonucleotides against a target of interest, such as a protein. The main goal of SELEX is to identify a small subset of aptamers from the original library that bind to the target of interest.

N6-methyl adenosine (N6-methyl ATP) is a base modified analog of adenosine and is found as a minor nucleoside in natural RNAs (Meyer et al.). N6-methyl ATP can substitute for ATP in some biological systems, and is a potent agonist for P2Y-purinoceptors in the guinea pig, taenia coli (Burnstock et al.). In vitro studies showed that N6-methyl ATP substituted for ATP and supported cytoskeletal filament-driven translocation of motor proteins (dynein, kinesin and myosin) (Schliwa et al. & Shimizu et al.). N6-methyl ATP is also a substrate for RNA polymerase and can be used for preparation of modified RNA (Rohayem & Kariko et al.).