Lentivirus

Please note this product may be subject to fees, we invite you to contact your local office. Vγ4Vδ1 TCR Lentivirus are replication incompetent, HIV-based, VSV-G-pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viruses transduce a TCR (T Cell Receptor) which belongs to the Vγ4Vδ1 subtype of γδ TCRsd. The TCR γ chain and δ chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

Please note this product may be subject to fees, we invite you to contact your local office. The Human CD4 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain CD4 (NM_000616.5) driven by an EF1a promoter, and a hygromycin selection marker.

The Influenza A Virus HA TCR Lentivirus (Clone HA1.7) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human TCR (T-cell receptor) clone HA1.7 that specifically recognizes the Influenza A virus (or IAV) HA (hemagglutinin) peptide (PKYVKQNTLKLAT) in the context of MHC (major histocompatibility complex) class II molecules. The Influenza A Virus HA TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

The Influenza A Virus HA TCR Lentivirus (Clone HA1.7) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human TCR (T-cell receptor) clone HA1.7 that specifically recognizes the Influenza A virus (or IAV) HA (hemagglutinin) peptide (PKYVKQNTLKLAT) in the context of MHC (major histocompatibility complex) class II molecules. The Influenza A Virus HA TCR α chain and β chain are linked by P2A. The lentiviruses also transduce a puromycin selection marker.

Please note this product may be subject to fees, we invite you to contact your local office. SRE TurboRFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an TurboRFP (red fluorescent protein) reporter driven by the SRE (Serum Response Element) located upstream of the minimal TATA promoter. The lentiviruses also transduce a hygromycin selection gene. After transduction, activation of the MAPK (mitogen-activated protein kinase)/ERK (extracellular regulated kinase) signaling pathway in target cells can be monitored by assessing RFP expression.

DLL1 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human DLL1 (delta like canonical notch ligand 1) (NM_005618.4), driven by a CMV promoter, and a puromycin selection marker.

DLL4 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles transduce cells with human DLL4 (delta like canonical notch ligand 4) (NM_019074.4), driven by a CMV promoter, and a puromycin selection marker.

GCaMP is a genetically encoded calcium indicator (GECI) and created from a fusion of green fluorescent protein (GFP), calmodulin (CaM), and M13, a peptide sequence from myosin light chain kinase. When GECI binds Ca2+, the conformational change of GCaMP induces proximity of the GFP parts, eliciting strong GFP fluorescence signal, which allows measuring action potentials and other receptor activation events that trigger Ca2+ influx. The advantage of GECI's are that they can be genetically specified for studies in living organisms. GCaMP6 is a novel ultra-sensitive format of GCaMP that outperformed other sensors in terms of accuracy and sensitivity in measuring cytosol Ca2+ level. Mutations in calmodulin part of GCaMP6 created several variants which showed different calcium fluorescence decay rate (fast - GCamP6f, slow - GCaMP6f and medium - GCaMP6m). LV-CaMKII-GCaMP6f is pre-made lentivirus which expresses GCaMP6f under CaMKII promoter for neuronal exclusive expression. Ready to us

Please note this product may be subject to fees, we invite you to contact your local office. Expression Negative Control Lentivirus (Inducible Tet On™) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These viral particles do not express any specific protein under a tight TRE tetracycline-inducible promoter but include a G418 selection marker.