Results for Antibodies ( 505272 )
Anti-Rabbit Secondary Antibody for CUTANA™ CUT&Tag is affinity purified and specific for rabbit immunoglobulins. Minimal cross reactivity with mouse, rat, human, bovine, guinea pig, and donkey IgG is observed. Use of a secondary antibody in CUT&Tag aids in signal amplification by increasing bound IgG per target epitope [1]. For best results, use with CUTANA™ pAG-Tn5 (EpiCypher 15-1017 & 15-1117). For detailed instructions for use, see EpiCypher’s CUTANA™ CUT&Tag protocol: epicypher.com/protocols. *Mixed Monoclonal: a pool of multiple recombinant monoclonal antibodies.
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SNF2H/SMARCA5 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the ISWI chromatin remodeler complex [1].
This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CTCF regulates transcription by binding DNA and preventing interaction between promoters and nearby enhancers or silencers.