6-plex Colorectal Cancer Methylation Panel - 4 boxes

The 6-plex PCR-ready assay is designed to profile DNA methylation in key regions of the MLH1 gene and its regulatory elements. This panel focuses on 5'-upstream regions, 5'-UTR, intron 1, and intron 14, with a total of 6 assays covering 26 CpG sites. Functional annotations include enhancers and promoters, with references to Encode accession numbers for added context. Functional Regions Covered: 1. 5'-Upstream (ADS8981, ADS820, ADS8973): Includes regulatory regions linked to CGI 93 and Encode Accession#: EH38E2192188. 2. 5'-UTR (ADS8984): Overlaps with CGI 93 and promoter regions (Encode Accession#: EH38E2192189). 3. Introns (ASY3464, ADS8987): Targets intron 1 and intron 14 with Encode Accession#: EH38E2192191. Additional Features: Includes analysis of the antisense strand of exon 1 of EPM2AIP1, providing insights into overlapping regulatory elements.