Cryo solution, 125 ml (serum-free) - 125 ml

In a serum-free cell culture system, it is very important to cryopreserve cells also in a serum-free freeze medium and not in a serum containing medium. Using an ordinary serum containing freeze medium, the serum affects the cell culture system over a long period. This influence can only be removed by dilution during a large number of passages after seeding. Provitro's Cryo-SFM is a new formulation for cryopreservation of animal and human cells containing no serum, but rather DMSO, methyl cellulose and other cryoprotectant ingredients. After cryopreservation and thawing, a very high percentage of viable cells is obtained. Cells which are cryopreserved in Cryo-SFM also show excellent attachment ability as well as growth performance after thawing. Adherent Cells: Remove adherent cells with Trypsin/EDTA (Provitro Cat.-No.: 204 0001). Neutralize Trypsin with Trypsin inhibitor (Provitro Cat.-No. 204 0001), pellet cells by centrifuging at low acceleration (200 x g) for 3-5 minutes and