Acidic Patch Mutant H2AE61A Recombinant Nucleosome with Lin - 50 µg

The acidic patch is a negatively charged region of the nucleosome surface that serves as a conserved interaction hub for neighboring nucleosomes and chromatin binding proteins, often via salt bridges with arginine anchors [1]. The acidic patch plays a critical role in chromatin condensation and chromatin remodeling [1-3]. Recombinant mononucleosomes containing acidic patch mutations can be used to study the biological functions of the acidic patch. H2AE61A Recombinant Nucleosome with Linker DNA consists of 199 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3.1, and H4) to form a nucleosome, the basic repeating unit of chromatin. The 5’ biotin-TEG DNA consists of a core 147 bp 601 nucleosome assembly sequence [4] flanked by 26 bp linker sequences as underlined below. Histone H2A contains a glutamate-to-alanine (E-to-A) substitution within the acidic patch at position 61 (H2AE61A). H2AE61 resides in the alpha2 helix, forming a key salt bridg