Optimizing RNA Therapeutics: Synthesis, Delivery, Expression
In today’s scientific landscape, advancing your research requires partnering with RNA experts. At Tebubio, our Contract Research Services (CRS) Laboratory delivers more than just RNA: we provide a complete, personalized service to support your therapeutic research goals.
Whether you're at the proof-of-concept phase or scaling up for clinical development, experience Tebubio’s tailored solutions from sequence design to in vitro and in vivo validation.
We guide you through every step to accelerate your journey, ensuring your research stands out at every stage.
RNA Mini-Scale Production: Accelerate Your RNA Research Studies
At Tebubio CRS we offer high-quality custom RNA production from 100 µg to 2 mg. Starting with sequence optimization, we enhance RNA stability and translation efficiency.
With options for specialized capping and modifications, we can reduce RNA immunogenicity, include fluorescent labels to monitor delivery or heavy atoms for NMR structural studies.
Whether using our proprietary plasmid or yours, we deliver RNA to your exact needs. Your template sequences are stored, to let you order new batches quickly and cost-effectively.
Automated production processes and rigorous quality controls ensures high purity and accuracy for every batch, helping you achieve reliable results.
RNA Delivery: Tailored Solutions for Your Research
Get your RNA ready for in vivo delivery by formulation into LNP systems. We encapsulate from 10 µg up to few mg RNA, with both standard and custom formulation tailored to your project needs.
We offer a range of off-the-shelf formulations, including for vaccines and CAR-T applications, as well as preferential delivery to specific organs: liver, lungs and spleen.
For custom compositions, we have access to an extensive lipid library through our partners: ionizable lipids, PEG-lipids, fluorescent lipids, and many more.
Additionally, we provide ready-to-use chemical transfection reagents optimized for both in vitro and in vivo systems, ensuring reliable results for your research.
Expression: Comprehensive In Vitro and In Vivo Validation
At Tebubio, we help you move beyond RNA production by providing robust in vitro and in vivo testing to generate meaningful biological data.
Explore our In Vitro Modeling service and discover our physiologically relevant cell models to assess the expression and therapeutic capacity of your RNA candidate.
Through our partners, we offer toxicity, biodistribution, and safety studies in several in vivo models (rodents, dog, monkey...).
Complementary Biomarker Mapping on samples from laboratory models and animal studies helps uncover the mechanism of action and biological processes your RNA is involved in.
For further studies, obtain your purified proteins of interest thanks to our extensive expertise in recombinant protein production and purification.
Why Tebubio Contract Research Services?
Our Contract Research Services give you a clear competitive edge by offering customized, high-quality mRNA production and testing solutions. Here’s why Tebubio stands out:
Solid Expertise in RNA
Our RNA Based Therapeutic Discovery platform has been awarded by the investment plan France 2030 for French technological competitivity.
Proven Track Record
With years of experience and over 300 successfully completed projects, we have the expertise you need for complex RNA-based research.
Integrative Solutions
Combine with our in vitro modeling and biomarker mapping services, providing a comprehensive approach to your research.
Seamless Transition to GMP Production
When you’re ready to move to large-scale production, we facilitate a smooth transition from research to GMP, ensuring that your project stays on track and ready for clinical development.
Dedicated PhD Project Manager
Your research project will be handled by a dedicated PhD-level expert who guides you every step of the way, ensuring your goals are met efficiently.
Projects 100% Handled in Europe
Every aspect of your project is managed right here in Europe, ensuring complete control and fast turnaround.
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Scientific Resources
Read our latest application notes, customer case studies and more.
Lipid Nanoparticules (LNP): The New Cargo for Drug Delivery
Explore how to optimize Lipid Nanoparticules for mRNA delivery for drug development.
This study highlights key formulation parameters: buffer composition, lipid selection; crucial for enhancing transfection and therapeutic efficacy.
Production of eGFP mRNA Labeled with Fluorophore for Tracking of Delivery and Expression
We produced fluorescently labeled eGFP mRNA to help assess LNP encapsulation efficiency and monitor mRNA delivery in mammalian cells.
Using Andy Fluor-UTP, we generated fluorescent mRNA detectable at multiple wavelengths and confirmed its successful transfection and expression in HEK293 cells.
Pricing
Starting from 740 €. Price depends on the length of your sequence of interest, as well as on the mRNA amount and modifications. Don't hestitate to get in touch to receive a personalized quotation.
Let's Collaborate to Advance Your Research
Our RNA Based Therapeutic Discovery Platform is designed to bring your research vision to life. Share the details of your project, and a dedicated PhD-level project manager will contact you to explore how we can tailor our solutions to your needs.
FAQ
Find answers to the questions most frequently asked by our users, curated by our experts.
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Can you provide mRNA coding for reporter genes?
Yes, we offer several off-the-shelf mRNAs for common reporter genes, including eGFP, mCherry, FLuc, GLuc. Other off-the-shelf mRNA proposed are OVA, and β-gal.
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I have an amino acid sequence for a protein of interest. Can you produce the corresponding mRNA?
Absolutely. We can design an mRNA sequence based on your protein’s amino acid sequence. We also perform codon optimization to minimize immunogenicity and reduce the risk of truncated mRNA forms.
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I already have a plasmid containing my sequence of interest. Can you produce mRNA from it?
Yes, we can synthesize mRNA directly from your plasmid, provided it is compatible with in vitro transcription. If not, we can insert your sequence into one of our optimized plasmids, featuring enhanced UTRs and polyA tails for efficient transcription and translation in mammalian cells.
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What are the minimum and maximum gene sizes for in vitro transcription (IVT)?
For IVT, the minimum gene size is 100 base pairs. While there is no strict upper limit, transcripts over 6 kb are more complex to produce and may result in lower yield consistency under standard conditions. Our high-resolution quality control system is also more effective for smaller transcripts.
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What quality control (QC) measures do you perform on LNPs?
We conduct Dynamic Light Scattering (DLS) to monitor LNP size and homogeneity (polydispersity index). We also assess encapsulation efficiency using mRNA quantification via Ribogreen Assay.
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What is the shelf-life of LNPs?
LNPs are stable for up to 3 weeks at 4°C. For extended storage, the addition of a cryoprotectant (sucrose or trehalose) enables LNPs to be kept at -20°C for several months.
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What transfection agents do you use for in vitro mRNA delivery?
For in vitro applications, we recommend using mRNA-fect or LNP formulations. mRNA-fect typically offers higher efficiency for in vitro delivery.
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Do you supply lipids directly?
Yes, as a distributor, we offer a broad selection of lipids and can provide custom lipids through our partners to meet your specific needs.