An Insight from the Tebubio Team
Accurately measuring cell viability is critical for drug screening, cancer research, and toxicity studies. However, many assays can be expensive or time-consuming.
The LIVE-Step™ Cell Assay System offers a fast, reliable, and cost-effective alternative, helping researchers streamline their workflows while maintaining high sensitivity.
A Simple and Reliable Way to Measure Cell Viability
Cell viability assays are essential in drug discovery, toxicology studies, and cancer research. Many existing solutions, however, come with challenges such as high costs, short signal stability, and complex workflows.
The LIVE-Step™ Cell Assay System from BPS Bioscience provides a sensitive and easy-to-use alternative for researchers looking to streamline their cell viability measurements. This ATP-based luminescence assay offers stable signals, high-throughput compatibility, and cost-effectiveness, making it a strong competitor to Promega’s CellTiter-Glo®.
Why Choose LIVE-Step™?
Accurately quantifying viable cells is crucial for evaluating drug effects, optimizing cell cultures, and studying disease models. Unlike traditional assays that require multiple steps and unstable luminescence signals, LIVE-Step™ provides:
- Simple, one-step protocol: Lyses cells and measures ATP levels in a single step.
- Enhanced sensitivity: Detects low cell densities with high accuracy.
- Stable signal: Maintains luminescence for over 5 hours, allowing flexibility in high-throughput screening.
- Cost-effective alternative: Priced lower than CellTiter-Glo® without compromising performance.
- Validated across multiple cell lines: Reliable results in both adherent and suspension cells.
How It Works
LIVE-Step™ measures ATP levels, a key indicator of metabolically active cells. The assay produces a luminescent signal that correlates directly with cell number, allowing researchers to assess cell viability and cytotoxicity with precision.
Its “add-and-read” format minimizes hands-on time, making it ideal for drug screening, toxicity testing, and cell proliferation studies.
Figure 1: Luciferin activity in the presence of ATP.
Figure 2: LIVE-Step™ Cell Assay System protocol overview.
Key advantages over CellTiter-Glo®:
- Lower cost per reaction.
- Longer signal stability, reducing variability in long-term experiments.
Who Should Use LIVE-Step™?
- Drug discovery researchers screening compounds for toxicity.
- Cancer biologists studying cell proliferation and apoptosis.
- Biomedical researchers optimizing cell culture conditions.
- Pharma and biotech labs performing high-throughput screening.
Whether working with tumor models, stem cells, or primary cultures, LIVE-Step™ provides a cost-effective and reliable solution for assessing cell viability.
Diana Perrier, Marketing Manager
Marketing Team at Tebubio
"When it comes to cell viability assays, researchers need a solution that is not only reliable but also easy to integrate into their workflows. The LIVE-Step™ Cell Assay System delivers just that—high sensitivity, long signal stability, and a simplified protocol. Whether you're studying cancer cells or screening drug candidates, this assay provides the precision and efficiency needed for consistent results."
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References
Article content created by Tebubio using courtesy materials provided by BPS Bioscience.
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