Development of Cost Effective and Robust 3D Colorectal Cancer Models for Physiological Drug Response Analysis

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Discover how robust and cost-effective 3D colorectal cancer models replicate physiological drug responses.

This poster showcases innovative platforms for preclinical research and drug development.

Abstract

Our customer needed to obtain in vivo like responses to drug treatment, through monitoring parameters such as gene expression, biomarker analysis, cellular viability, invasion capability.

We established and implemented a physiologically relevant 3D Colorectal cancer cell-based assay. To answer questions related to variations at transcription and protein level, our methodology required long-term imaging at controlled temperature and gas levels (CO2 and O2), on both 3D cellular models and patient-derived samples.

Impact of an anti-cancerous drug was investigated on colon cell lines (HT-29, HCT116 and LS174T) in conventional 2D monolayer and 3D spheroid cultures, in hypoxia or normoxia. Methods used were metastatic invasion assay, gene expression analysis by RT-PCR on hypoxic response and oncopathway genes, and large-scale protein profiling to detect proteomic levels of metalloproteinases and surface proteins known to be involved in colorectal cancer invasion.

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  • Authors

    Dora Sabino, Isabelle Fixe, Alexandra Foucher, Eric Mennesson, Nadia Normand

  • Affiliation

    Tebubio - 39 rue de Houdan - 78612 Le Perray-en-Yvelines - France

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