An Insight from the Tebubio Team
Discover how SPY555-Golgi, SPY650-Golgi, Halo-Flipper, and the CA-based HaloTag substrates are revolutionising live-cell imaging. These cutting-edge probes offer researchers unparalleled precision ensuring reliable and efficient microscopy workflows.
Enhance your imaging studies with Spirochrome’s latest innovations, designed to streamline experimental processes and unlock new possibilities in cell biology.
As the complexity of cellular research grows, scientists need tools that are precise, versatile, and easy to use. To meet these demands, Spirochrome has expanded its portfolio with a series of innovative products designed to enhance fluorescence microscopy workflows.
These cutting-edge probes from our trusted supplier: Spirochrome, are now available on our website and empower researchers to tackle imaging challenges with confidence.
Why Researchers Choose Spirochrome Probes
Fluorescence imaging is a cornerstone of cellular biology, but it often comes with hurdles like low specificity, photobleaching, and limited compatibility across experimental setups. Spirochrome’s latest offerings address these challenges with:
- Bright and photostable fluorophores for consistent imaging.
- Fast and efficient labelling to save valuable time.
- Compatibility with advanced imaging platforms, including confocal, widefield, and super-resolution techniques.
These features help researchers focus on their discoveries, not the limitations of their tools.
Introducing Spirochrome’s New Probes
CA-Based HaloTag Substrates
For labs using HaloTag fusion proteins, Spirochrome’s ChloroAlkane (CA) substrates offer unmatched flexibility. These substrates permanently label proteins of interest, allowing precise visualisation in both live and fixed cells.
- SPY505-CA: Ideal for green channel imaging (512/532 nm), providing bright fluorescence with minimal background.
- SPY555-CA: Optimised for orange channel imaging (555/580 nm), perfect for detailed protein localisation studies.
- CPY-CA: A versatile cyan probe (490/515 nm) for experiments requiring additional colour separation.
- SiR-CA: Delivers exceptional far-red imaging (652/674 nm) with low phototoxicity.
- SPY700-CA: Designed for near-infrared imaging (700/720 nm), minimising autofluorescence and improving signal-to-noise ratio.
Each substrate offers rapid labelling kinetics and compatibility with super-resolution techniques like STED and SIM microscopy. For added convenience, the CA_TestKit combines SPY505-CA, SPY555-CA, and SiR-CA in one kit, catering to diverse experimental needs.
Halo-Flipper: Monitoring Membrane Tension
Understanding membrane dynamics is crucial for studying processes like endocytosis and cell motility. The Halo-Flipper probe is a specialised tool for measuring membrane tension in live cells. Its sensitivity to lipid bilayer dynamics offers insights into cellular mechanics that are otherwise difficult to obtain.
Golgi-Specific Probes: SPY555-Golgi and SPY650-Golgi
Visualising organelles with specificity is a common challenge in cell biology. The SPY555-Golgi (555/580 nm) and SPY650-Golgi (652/674 nm) dyes are designed to label the Golgi apparatus selectively. These probes are ideal for studying protein sorting, trafficking, and Golgi dynamics in live or fixed cells. Their bright fluorescence and stability ensure reliable results across various microscopy platforms.
Figure 1: Spirochrome's Golgi probe.
Customer-Centric Features: Meeting Your Needs
Researchers face distinct challenges when choosing imaging reagents. Spirochrome’s new products are designed to address these needs directly:
- Efficiency for High-Throughput Studies: The fast labelling kinetics of CA substrates and Golgi dyes reduce preparation time, enabling rapid experimental workflows.
- Versatility Across Techniques: Whether conducting widefield imaging or exploring super-resolution methods, these probes integrate seamlessly into your setup.
- Cost-Effective Options: Products like the CA_TestKit provide multiple fluorophores in one package, maximising flexibility while minimising expense.
- Minimised Phototoxicity: The far-red and near-infrared options (SiR-CA, SPY700-CA) ensure minimal impact on live-cell health, critical for time-lapse studies.
Experience the Spirochrome Advantage
With Spirochrome’s innovative probes, you can elevate your research to new heights. From dynamic membrane studies to organelle-specific labelling and protein visualisation, these tools are tailored to help you achieve accurate, reproducible results.
Agnès Marcilly, MSc
Marketing Team at Tebubio
"Spirochrome’s latest probes aren’t just fluorescent dyes; they’re tools designed to make microscopy more efficient, reliable, and accessible to every scientist striving for breakthrough discoveries."
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References
Figure 1: Courtesy of Spirochrome AG, your trusted supplier for innovative imaging solutions.
Ready to take your cell imaging to the next level?
Contact us today to learn more about Spirochrome's new probes or to request a quote.