An Insight from the Tebubio Team
Discover how PKmem™ 555, PKmem™ 590 and PKmem™ 650 probes redefine live cell imaging by specifically and efficiently labelling plasma membranes, offering researchers unparalleled accuracy and ease of use.
Improve your cellular studies with cutting-edge tools and transform your approach to plasma membrane research to unlock new possibilities in cell biology.
Introducing PKmem™ Probes: Crossing Cellular Boundaries with Precision
In cellular research, precisely visualising the plasma membrane can unlock critical insights. However, traditional labelling techniques often present challenges like phototoxicity, low specificity, or complex workflows. Spirochrome’s PKmem™ 555, PKmem™ 590, and PKmem™ 650 probes offer a transformative solution, addressing these limitations and empowering researchers to confidently explore cellular dynamics.
Designed for simplicity, brightness, and cellular safety, these probes are ideal for applications ranging from neurobiology to cancer research. Let’s delve into how they elevate plasma membrane imaging.
Precision and Versatility Tailored to Researchers’ Needs
At the core of PKmem™ probes’ effectiveness is their exceptional specificity for plasma membranes in live cells. Unlike traditional fluorescent proteins or dyes requiring genetic manipulation, PKmem™ 555, PKmem™ 590, and PKmem™ 650 provide a seamless, ready-to-use alternative. Researchers can achieve vivid membrane labelling without additional preparation steps, simplifying experimental workflows.
The probes are compatible with a variety of microscopy techniques, including widefield, confocal, and structured illumination microscopy (SIM). With fluorescence profiles spanning different channels, they offer flexibility in multicolour imaging protocols:
- PKmem™ 555: Absorbs at 556 nm and fluoresces at 572 nm, ideal for red-channel imaging.
- PKmem™ 590: Bridges the spectrum between PKmem™ 555 and PKmem™ 650, enhancing imaging options and enabling versatile experimental designs.
- PKmem™ 650: Extends into the far-red spectrum for deeper tissue penetration and reduced background interference.
Video 1: Confocal imaging of brain slices stained with PKmem™ and Hoechst.
Safer, Brighter, and Built for Longevity
One of the standout innovations in PKmem™ probes is their incorporation of cyclooctatetraene (COT), a triplet quencher that dramatically reduces phototoxicity. This feature enables extended live cell imaging sessions without compromising cellular viability—critical for studying long-term processes like membrane trafficking, intercellular communication, or therapeutic interventions.
Additionally, their high brightness ensures exceptional visual clarity, even under low-intensity light, preserving cells while maintaining signal integrity.
Key Advantages at a Glance
- Non-Toxic and Photostable: Ensures minimal impact on cell health, even during prolonged imaging sessions.
- High Specificity: Targets plasma membranes exclusively, avoiding artefacts or background fluorescence.
- No Genetic Modification: Eliminates the need for transfection or protein overexpression, saving time and reducing variability.
- Multicolour Imaging Compatibility: Works seamlessly with fluorophores like SPY dyes and GFP.
Wide Application Range: Suitable for diverse cell types and imaging techniques, from standard to advanced setups.
Figure 1: Mouse Embryonic Stem Cells (mESC) PKmem™ 555 & PKmito deep red montage.
Your Solution for Advanced Plasma Membrane Imaging
For researchers seeking to improve the reliability and efficiency of their experiments, PKmem™ 555, PKmem™ 590, and PKmem™ 650 represent a leap forward. Whether you're examining cellular organisation, studying disease mechanisms, or testing new drug candidates, these probes enable precise, reproducible, and non-invasive imaging tailored to the demands of modern science.
Agnès Marcilly, MSc
Marketing Team at Tebubio
"I believe that tools like PKmem™ probes are more than just innovations—they are enablers of discovery, offering precision and simplicity that empower scientists to push the boundaries of cellular imaging."
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References
Video 1 and figure 1: Courtesy of Spirochrome AG, your trusted supplier for innovative imaging solutions.
Discover the Difference Today
Unleash the potential of your research with Spirochrome's PKmem™ probes. Explore the full capabilities of these innovative tools and transform your imaging workflow. Contact us to request a quote or place your order.