Standardized 3D Spheroid Systems Facilitate Drug Screening and Biomarker Identification with High Relevance for Cancer Drug Discovery

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Evaluating the impact of key cell-culture parameter modulation on the predictivity for the identification of specific inhibitors and biomarkers on cancer models.

Abstract

Our customer needed to investigate whether modulating key culture parameters could improve the predictivity of their colorectal cancer cell-based methodology used to select CDK8 inhibitors and/or identify biomarkers.

Tebubio Contract Research Services's laboratory performed comparative analysis for them, which involved developing two HT-29 colorectal cancer models: as monolayers in a conventional incubator and as 3D spheroids under physioxia, in the presence/absence of gamma-interferon. On each model, a viability assay was performed after 3 days incubation time with inhibitors, followed by large-scale proteome profiling analysis on 2000 targets from each cell lysates.

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  • Authors

    Julien Duez1 - Isabelle Fixe1 - Alexandra Foucher1 - Eric Mennesson1 - Nadia Normand1

    Zhizhou Kuang2 - Jingqiao Lu2

  • Affiliations

    1Tebubio - 39 Rue de Houdan - Le Perray en Yvelines - France

    2RayBiotech Inc. - 3607 Parkway Lane, Suite 200 - Norcross, GA 30092 - USA

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