H2DCFDA (2',7'-dichlorodihydrofluorescein diacetate) - 250 MG

H2DCFDA is a cell-permeant indicator used to detect reactive oxygen species (ROS) in living cells. Initially non-fluorescent, it becomes highly fluorescent after intracellular esterases remove its acetate groups and oxidation occurs. The resulting product is 2’,7’-dichlorofluorescein (DCF), which emits bright green fluorescence with an absorption maximum at 511 nm and a fluorescence maximum at 533 nm. It’s commonly employed for ROS assays in living cells and is not suitable for sample fixation.