20X SSC (3.0 M Sodium Chloride, 0.3 M Sodium Citrate, pH 7.0 - 4 x 1 L

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridization process and washing in experiments where species of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Northern blotting, in situ hybridization, or DNA microarrays). SSC is used to help control the stringency of hybridization in these steps.