2X SDS-PAGE Sample Buffer without DTT or b-ME (125 mM Tris, - 100 mL

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearize proteins and to impart a negative charge to linearized proteins. Visit our newly expanded web site at www.rockland.com for methods using this and other buffers.