*Mouse Total Urokinase (uPA) ELISA Kit - 1kit

Mouse uPA total antigen assay is intended for the quantitative determination of total plasminogen activator antigen in mouse plasma. For research use only. Strip well format. Reagents for up to 96 tests. Mouse uPA will bind to the capture antibody coated on the microtiter plate. Free and complexed enzyme will react with the capture antibody on the plate. After appropriate washing steps, polyclonal anti-murine uPA primary antibody binds to the captured enzyme. Excess antibody is washed away and bound polyclonal antibody is then reacted with the secondary antibody conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of uPA.