SH2-Domain containing inositol 5-phosphatases (SHIP1 & SHIP2) dephosphorylate the 5-position of PI(3 4 5)P3 generating PI(3 4)P2. SHIP2 is ubiquitously expressed while SHIP1 is found in hematolymphoid cells and mesancymal stem cells. K118 is a water soluble SHIP1 inhibitor which does not inhibit SHIP2. Treatment with K118 (10 mg/kg) on obese mice on a high fat diet resulted in reduced body mass and lowered fat content when compared to pre-treatment and non-treated control animals. The treated mice had reduced blood glucose and insulin levels in addition to improved glucose tolerance. K118 treatment also reduced the amount of age-related fat accumulation in mice.

The Acid Sphingomyelinase Assay Kit is an enzyme assay that measures acid sphingomyelinase activity in biological samples through the direct hydrolysis of a fluorogenic substrate.Assay sensitivity: 25 pmolSample Type: tissue homogenates cell lysatesSample Volume: 50 µL/wellProduct BackgroundThe  Acid Sphingomyelinase Assay Kit utilizes a fluorogenic substrate that is specific for acid sphingomyelinase to provide a sensitive and homogenous method to measure the activity of aSMase in vitro. The kit provides all necessary reagents to measure the acid sphingomyelinase activity of 40 samples ran in duplicate.Sphingomyelinases catalyzes the hydrolysis of sphingomyelin into ceramide and phosphoryl choline; and is involved in programmed cell death (apoptosis) cell differentiation and cell proliferation. Sphingomyelinases are classified into five categories: acid sphingomyelinase (aSMase) secretory sphingomyelinase (sSMase) neutral Mg 2 +/- dependent sphingomyelinases (nSMase) neutral

The PI(3)P Mass ELISA measures the amount of PI(3)P extracted from cells by means of a competitive ELISA.Assay Range: 400 to 0.39 pmol PI(3)PSample Type: Dried down lipid extracted from cellsSample Volume: 180 µL/sample run in triplicate.Product BackgroundThe PI(3)P Mass ELISA Kit a 96-well competitive ELISA allows for the determination of PI3-K activity by quantifying the amount of PI(3)P found in cells. After following a non-radioactive lipid extraction protocol the PI(3)P samples obtained from millions of cells are detected on a competitive ELISA. The cellular PI(3)P samples are mixed and incubated with a PI(3)P Detector protein then added to a PI(3)P-coated microplate for competitive binding. A peroxidase-linked secondary detector and colorimetric detection is used to detect the PI(3)P protein binding to the plate. The assay is sensitive to 1.0 pmol PI(3)P. The colorimetric signal is inversely proportional to the amount of PI(3)P obtained.Keywords: PI3K PI3 Kinase PI3 K PI

Phosphatidylinositol 4 5-bisphosphate diC8 (PI(4 5)P2 diC8) is a synthetic purified dioctanoyl PI(4 5)P2.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 4 5-bisphosphate (PIP2) has been shown to play a central role in a variety of cellular functions. Amongst its many functions PIP2 is a substrate for Phospholipase C-coupled G-protein pathways involved in intracellular calcium release in a number of tissues and is also a substrate for class I phosphoinositide 3-kinase (PI3-K).Alternate Names: D-myo-Phosphatidylinositol 4 5-bisphosphate Dioctanoyl Phosphatidylinositol 4 5-bisphosphate PtdIns(4 5)P2 C8 PI(4 5)P2 C8 or PIP2Bulk discounts available please email echelon@echelon-inc.com for information.Publications Powered by Bioz See more details on Bioz1) Jenco J. M. A. Rawlingson et al. (1998). "Regulation of phospholipase D2: selective inhibition of mammalian phospholipase

Shuttle PIP kits are designed for researchers interested in visualizing and localizing intracellular phosphoinositides. These kits contain fluorescent and non-fluorescent phosphoinositides carriers and fluorescent-labeled carriers. Two-color studies are possible using BODIPY® FL labeled PIPs and tetramethylrhodamine (TMR)-labeled carriers.Kit ContentsPhosphoinositidesPI(3 4)P2diC16BODIPY FL-PI(3 4)P2CarriersNeomycin SulfateNeomycin-TMRHistone H1Histone H1-TMRCarrier 3

Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein proteins in cell lysate or binding of radiolabeled in vitro translation products.Control Beads are blocked agarose beads for use as a negative control with Lipid Coated Beads.Storage4 °C - do not freeze Powered by Bioz See more details on Bioz

Lipid coated beads are designed for use in protein pull-down experiments. Use to test for lipid binding of purified protein proteins in cell lysate or binding of radiolabeled in vitro translation products.Phosphatidic Acid Beads are composed of agarose with 10 nanomoles of bound phosphatidic acid (PA) per 1ml of beads enough for several protein binding experiments. Each 1 mL order of Phosphatidic Acid Beads includes a small amount of control beads. Larger quantities of Control Beads are also available for purchase.Storage4 °C - do not freeze Powered by Bioz See more details on Bioz

Farnesoic acid ((2E 6E)-3 7 11-trimethyldodeca-2 6 10-trienoic acid) is the substrate of farnesoic acid O-methyltransferase which produces the crustacean reproductive hormone methyl farnesoate (MF). MF is responsible for enhancing reproductive maturation maintaining juvenile morphology and influencing male sex determination. Farnesoic acid also inhibits filiment formation in C. albicans. Powered by Bioz See more details on BiozFeatured in Publications1) Li Y. G. C. Unnithan et al. (2003). "Stimulation of JH biosynthesis by the corpora allata of adult female Aedes aegypti in vitro: effect of farnesoic acid and Aedes allatotropin." J Exp Biol 206(Pt 11): 1825-32.2) Zhao N. J. Guan et al. (2007). "Molecular cloning and biochemical characterization of indole-3-acetic acid methyltransferase from poplar." Phytochemistry 68(11): 1537.3) Chung S.-C. T.-I. Kim et al. (2010). "Candida albicans PHO81 is required for the inhibition of hyphal development by farnesoic acid." FEBS letter

Methyl farnesoate (MF) is a crustacean reproductive hormone that is structurally similar to insect juvenile hormone. It is responsible for enhancing reproductive maturation maintaining juvenile morphology and influencing male sex determination. Exposure of female Daphnids to increasing levels of MF increases the percentage of males in a brood in a dose-dependant manner. MF is endogenously produced in the mandibular organ and environmental factors such as salinity and temperature can influence hemolyph levels. Powered by Bioz See more details on BiozFeatured in Publications1) Olmstead A. W. and G. A. LeBlanc (2007). "The environmental-endocrine basis of gynandromorphism (intersex) in a crustacean." Int J Biol Sci 3(2): 77-84.2) Eads B. D. J. Andrews et al. (2007). "Ecological genomics in Daphnia: stress responses and environmental sex determination." Heredity 100(2): 184.3) Nagaraju G. P. C. and D. W. Borst (2008). "Methyl farnesoate couples environmental changes to testicular d