Products

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. SNF2H/SMARCA5 antibody produces CUT&RUN peaks above background (Figure 1) that overlap with H3K4me3 (Figures 1-2), consistent with its known role as the ATP-dependent helicase subunit of the ISWI chromatin remodeler complex [1].

This antibody meets EpiCypher’s “CUTANA Compatible” criteria for performance in Cleavage Under Targets and Release Using Nuclease (CUT&RUN) and/or Cleavage Under Targets and Tagmentation (CUT&Tag) approaches to genomic mapping. Every lot of a CUTANA Compatible antibody is tested in the indicated approach using EpiCypher optimized protocols (epicypher.com/protocols) and determined to yield peaks that show a genomic distribution pattern consistent with reported function(s) of the target protein. CTCF regulates transcription by binding DNA and preventing interaction between promoters and nearby enhancers or silencers.

The CUTANA™ ChIC/CUT&RUN Kit enables streamlined chromatin profiling of histone post-translational modifications (PTMs) and chromatin associated proteins while providing increased throughput and reproducibility with multi-channel pipetting. Positive (H3K4me3) and negative (IgG) control antibodies are included to pair with SNAP-CUTANA™ spike-in controls for assay optimization and continuous monitoring (Figure 2). E. coli DNA is included for data normalization. The kit is compatible with a variety of inputs including cells or nuclei derived from native, cryopreserved, or cross-linked samples. While it is recommended to start with 500,000 cells, comparable data can be generated using as few as 5,000 cells. The inclusion of controls, as well as compatibility with diverse target types, sample inputs, and low cell numbers, make this kit the go-to solution for chromatin mapping experiments.

CUTANA pAG-Tn5 is the key reagent for performing efficient mapping of chromatin features in Cleavage Under Targets and Tagmentation (CUT&Tag) [1]. CUT&Tag offers significant improvements in signal to noise at reduced cell inputs and sequencing depth compared to ChIP-seq. As a fusion of Proteins A and G to a highly active E. coli transposase mutant (Tn5), CUTANA pAG-Tn5 is compatible with target antibodies from a broad spectrum of host species. It also comes without an epitope tag, making it suitable for tag-mediated CUT&Tag (e.g. FLAG, HA, TY1, V5, etc.). This product is highly purified to remove contaminating E. coli DNA, which can be tagmented during storage and complicate analysis at low cell numbers. For superior normalization as well as antibody validation and reaction monitoring, SNAP-CUTANA™ nucleosome spike-ins (e.g. EpiCypher 19-1002) are recommended. The Tn5 comes charged with mosaic adapters and is ready to be used in CUT&Tag.

Human mononucleosomes purified from HeLa cells. The nucleosome is the basic repeating unit of chromatin, which consists of 150 base pairs of DNA wrapped around an octamer core of histone proteins (two each of H2A, H2B, H3, and H4).

This product contains Concanavalin A (ConA) conjugated to paramagnetic microspheres. ConA is a lectin (carbohydrate-binding protein) that binds specifically to mannosyl- and glucosyl-containing extracellular glycoproteins. The ConA magnetic beads are therefore useful to immobilize cells or nuclei presenting these glycans in their extracellular matrices.

ELISA Kit for Cathepsin S (CTSS)

SiR-actin is based on the F-actin binding natural product jasplakinolide. SiR-actin is fluorogenic, cell permeable and highly specific for F-actin. Sir-actin stains endogenous F-actin without the need for genetic manipulation or overexpression. Its emission in the far red minimizes phototoxicity and sample autofluorescence. SiR-actin is compatible with GFP and/or m-cherry fluorescent proteins. It can be imaged with standard Cy5 filtersets. SiR-actin can be used for widefield, confocal, SIM or STED imaging in living cells and tissue. Probe quantity allows 50 – 200 staining experiments.