Cell-based Assays

The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix. CheKine™ Micro Plant Root Vitality (TTC-Method) Assay Kit can be used to detect biological samples such as plant tissues. In this kit, The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenyl formazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorption peak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.

Sucrose is not only an important photosynthetic product but also the primary substance transported within plants and serves as a form of carbohydrate storage. Sucrose phosphate synthase (SPS, EC 2.4.1.14) uses fructose-6-phosphate as a receptor, and the formed sucrose-6-phosphate is converted into sucrose by sucrose phosphate phosphatase. The SPS-sucrose phosphate phosphatase system is generally considered the main pathway for sucrose synthesis. CheKine™ Mirco Sucrose Phosphate Synthase (SPS) Activity Assay Kit provides a simple, convenient, and rapid method for detecting SPS activity, suitable for plant tissue samples. The principle involves SPS catalyzing the formation of sucrose-6-phosphate from fructose-6-phosphate. Sucrose-6-phosphate reacts with resorcinol to produce a color change, exhibiting a characteristic absorption peak at 480 nm, with the intensity of the color being proportional to the level of enzyme activity.

Sucrose is the primary form in which photosynthetic products from sources (such as leaves) are transported to “sink” organs. Sucrose synthase (EC 2.4.1.13) is a reversible enzyme that can catalyze both the synthesis and breakdown of sucrose, making it a key enzyme in sucrose metabolism. Studying the activity of its invertase direction (SS-I) is significant for understanding plant sucrose degradation and starch synthesis. CheKine™ Mirco Sucrose Synthase-I (SS-I) Activity Assay Kit offers a simple, convenient, and rapid approach for detecting sucrose synthase (SS-I) activity, suitable for plant tissue samples. The principle of the kit involves SS-I catalyzing the reaction between sucrose and UDP to produce free fructose and UDPG; the content of reducing sugars is then measured using the 3,5-dinitrosalicylic acid method to reflect the level of enzyme activity

Sucrase (EC 3.2.1.26) is one of the key enzymes for carbohydrate digestion and absorption, capable of hydrolyzing sucrose into monosaccharides that can be absorbed by the body. CheKine™ Mirco Sucrase Activity Assay Kit offers a simple, convenient, and rapid approach for assessing sucrase activity, which is suitable for plant tissue samples. The principle is that 3,5-dinitrosalicylic acid, when heated with reducing sugars, is reduced to a brown-red amino compound, and within a certain range, the quantity of reducing sugars is proportional to the color intensity of the reaction solution. This method is easy to perform, quick, and has minimal interference from impurities.

Detect SA-β-Gal levels, Compatible with co-staining with immunostaining, Enable multiple staining with common green and red dyes

The Fc (IgG1): FcRn Inhibitor Screening Colorimetric Assay Kit is designed for the screening and profiling of neutralizing antibodies or inhibitors of the interaction between human Fc (IgG1) and human FcRn (Neonatal Fc receptor for IgG). This kit comes in a convenient 96-well or 384-well format, with purified Biotinylated-FcRn complex (Fc receptor amino acids 24-297 and B2M amino acids 21-119) and Fc (IgG1) (amino acids 100-330) proteins, Streptavidin-HRP, and assay buffers for 100 or 400 reactions. A 96-well plate is coated with Fc (IgG1) protein. After blocking, the plate is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with FcRn-biotin, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader. The signal is proportional to the binding of FcRn to Fc (IgG1).

The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN Helicase Activity Assay Kit comes in a convenient 384-well format, with contains enough purified recombinant WRN, ATP, DNA substrate, assay buffer and additives for 400 reactions. The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.

The PDE6C Assay Kit is a fluorescence polarization (FP), homogeneous, 384-well assay kit designed for the screening and profiling of PDE6C (Phosphodiesterase 6C) inhibitors. This assay takes advantage of a specific fluorescent phosphate-binding nanoparticle. The kit contains enough purified recombinant PDE6C, fluorescent probe, PDE assay buffer, Binding Agent, and diluent for 400 reactions. The assay uses a fluorescein-labeled cyclic guanosine monophosphate (cGMP-FAM for PDE6C), in which the phosphate group is engaged within the cyclic nucleotide. This is a very small molecule that rotates fast (low FP). PDE6C catalyzes the hydrolysis of the phosphodiester bond in the cyclic nucleotide and frees the phosphate group. In a second step the free phosphate group is recognized by a specific phosphate-binding nanobead (Binding Agent) leading to the formation of a large complex, with restricted movement (high FP). FP is proportional to PDE6C activity. This assay requires a fluorescent micropl

The FcRn:HSA Binding Chemiluminescent Assay Kit is designed for screening and profiling neutralizing antibodies or blockers of the interaction between Human Serum Albumin (HSA) and human FcRn. This kit comes in a convenient 384-well format, with Biotinylated HSA and FcRn (FCGRT/B2M) (amino acids 24-297 of FCGRT and 21-119 of B2M), Streptavidin-HRP, and assay buffers for 400 reactions. The assay requires only a few steps. First, FcRn is coated on a 384-well plate overnight. After blocking, the protein is pre-incubated with the neutralizing antibody or blocker. Upon subsequent incubation with Biotin-HSA, the plate is treated with Streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.