Functional Assays

The Chemi-Verse™ TNIK Kinase Assay Kit is designed to measure TNIK (TRAF2 and NCK interacting kinase) serine-threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified TNIK, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ ULK1 Kinase Assay Kit is designed to measure ULK1 (Unc-51-like autophagy-activating kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified ULK1, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ Wee1 Kinase Assay Kit is designed to measure Wee1 serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified Wee1 (amino acids 215-646(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Human IgG Chemiluminescent ELISA Kit is designed to detect and quantify protein levels of human captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated IgG-specific antibody plate, enough recombinant purified IgG1 standard, and detection reagents for 100 wells.

The IgG Colorimetric ELISA Kit is designed to detect and quantify protein levels of human IgG captured by pre-matched antibody pairs. This assay kit comes in a convenient 96-well format, with a pre-coated IgG-specific antibody plate, enough recombinant purified IgG1 standard, and detection reagents for 100 wells.

Triose-phosphateisomerase (TPI) in plant chloroplasts is an important enzyme involved in calvin cycle in photosynthesis. Acting on the transformation between glyceraldehyde phosphate and dihydroxyacetone phosphate, dihydroxyacetone phosphate can quickly penetrate the chloroplast envelope and enter the cytoplasm, where it is gradually transformed into sucrose. CheKine™ Micro Triose-Phosphate Isomerase (TPI) Activity Assay Kit can detect plant tissues In this kit, TPI converts dihydroxyacetone phosphate into glyceraldehyde-3-phosphate, and glyceraldehyde-3-phosphate and NAD react with glyceraldehyde-3-phosphate dehydrogenase to generate glyceric acid-3-phosphate and NADH. The absorbance change at 340 nm reflects the activity of TPI.

β-Xylosidase (EC 3.2.1.37) exists in organisms such as plants, bacteria, and fungi. It is a key enzyme that catalyzes the degradation of xylan-based hemicelluloses, and the product xylose can serve as a carbon source for microbial fermentation. Additionally, β-xylosidase can be used as a biobleaching agent in the paper industry, offering environmental benefits over traditional bleaching methods and possessing broad application value. CheKine™ Mirco β-Xylosidase (β-X) Activity Assay Kit offers a simple, convenient, and rapid approach for assessing β-Xylosidase activity, which is suitable for plant tissue, bacteria, and fungi samples. The principle involves β-xylosidase catalyzing the production of p-nitrophenol from p-nitrophenyl-β-D-xyloside. p-Nitrophenol exhibits a characteristic absorption peak at 405 nm. By measuring the rate of increase in absorbance at 405 nm, the β-xylosidase activity can be calculated.

Fructose -1,6- diphosphatase (FBP), also known as fructose -1,6- diphosphatase, plays a key role in gluconeogenesis and the synthesis of sucrose, an assimilate of photosynthesis. Catalyze fructose-1,6- diphosphate and water to produce fructose-6- phosphate and inorganic phosphorus. CheKine™ Micro Fructose 1,6-Bisphosphatase (FBP) Activity Assay Kit can detect animal and plant tissues, cells, plasma, serum or other liquid samples. In this kit, FBP catalyzes fructose-1,6-diphosphate and water toproduce fructose-6-phosphate and inorganic phosphorus. Glucose-6-phosphate isomerase and glucose-6-phosphate dehydrogenase added in the reaction system catalyze glucose-6-phosphate and NADPH in turn, and the activity of FBP can becalculated by measuring the increase rate of NADPH at 340 nm.

Fructose 1,6-diphosphate aldolase (FBA)(EC.4.1.2.13) is an important enzyme involved in calvin cycle in glycolysis, gluconeogenesis, pentose phosphate pathway and photosynthesis, which catalyzes the reversible cleavage of fructose 1,6-diphosphate into dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, and is widely used. CheKine™ Micro Fructose 1,6-Bisphosphate Aldolase (FBA) Activity Assay Kit can detect animal and plant tissues, cells, fungus, plasma, serum or other liquid samples. In this kit, FBA catalyzes fructose-1,6-diphosphate to produce glyceraldehyde-3-phosphate and dihydroxyacetone-3-phosphate, and NADH and dihydroxyacetone-3-phosphate are catalyzed to produce NAD and α-glycerophosphate under the action of triose phosphate isomerase and α-glycerophosphate dehydrogenase. The change of absorbance at 340 nm can reflect the activity of FBA.