Functional Assays

The Chemi-Verse™ c-MET Kinase Assay Kit is designed to measure c-MET (mesenchymal-epithelial transition) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified c-MET (amino acids 956-1390), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ GSK3α Kinase Assay Kit is designed to measure GSK3α (Glycogen Synthase Kinase 3 Alpha) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified GSK3α, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ GSK3β Kinase Assay Kit is designed to measure GSK3β (Glycogen Synthase Kinase 3 Beta) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified GSK3β, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ ROS1 Kinase Assay Kit is designed to measure ROS1 (ROS Proto-Oncogene 1) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified ROS1 kinase domain (amino acids 1883-2347(end)), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ RET Kinase Assay Kit is designed to measure RET (rearranged during transfection) tyrosine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified RET (amino acids 658-end), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ HPK1 Kinase Assay Kit is designed to measure HPK1 (Hematopoietic progenitor kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified HPK1 (amino acids 1-346, kinase domain), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions. Note: MBP (Myelin Basic Protein) is a non-specific protein substrate that is used as a "universal substrate" for many in vitro kinase activity assays. This protein is targeted by many serine/threonine kinases at conserved amino acids. We use the dephosphorylated version of the MBP substrate in our assays to determine the kinase-mediated phosphorylation of MBP. Our assays are not suitable for studying autophosphorylation of the kinase due to the presence of the MBP substrate.

The Chemi-VerseTM STK3 (MST2) Kinase Assay Kit is designed to measure STK3 (serine/threonine-protein kinase 3), also known as MST2, tyrosine kinase activity for screening and profiling applications using ADP-GloTM as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified STK3, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-VerseTM TYK2 Kinase Assay Kit is designed to measure TYK2 (Tyrosine Kinase 2) tyrosine kinase activity for screening and profiling applications using ADP-GloTM as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified TYK2, kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.

The Chemi-Verse™ MINK1 Kinase Assay Kit is designed to measure MINK1 (misshapen/NIK-related kinase 1) serine/threonine kinase activity for screening and profiling applications using ADP-Glo™ as a detection reagent. The assay kit comes in a convenient 96-well format, with enough purified MINK1 (amino acids 1-320), kinase substrate, ATP, and kinase assay buffer for 100 enzyme reactions.