Functional Assays

Malic enzyme (ME) is widely present in the cytoplasm of microorganisms, cultured cells, animals, and plants, especially with high activity in plant tissues. ME catalyzes the reversible oxidation decarboxylation of malic acid, producing pyruvate and CO2, as well as the reduction reaction accompanied by NAD(P)+, which is a key enzyme in malic acid metabolism. ME activity is closely related to biosynthesis and antioxidant activity. Based on the specificity of coenzyme and substrate, ME can be divided into NAD-ME (EC1.1.1.38) and NADP-ME (EC1.1.1.40). NAD-ME can catalyze the reduction of NAD+ to NADH, and the rate of NADH increase at 340 nm can reflect its activity.

SucrosePhosphorylase (SP, EC2.4.1.7) is mainly present in microorganisms and plants and belongs to the glycosylhydrolase 13 family. It is an enzyme that catalyzes the transfer of glucoside bonds and can catalyze the synthesis of 1-phospho-glucose from sucrose and inorganic phosphate. This enzyme mainly uses sucrose and glucose 1-phosphate as donors, and many substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyl groups, carboxyl groups as receptors to catalyze the synthesis of various glycosides. CheKine™ Micro Sucrose Phosphorylase (SP) Activity Assay Kit can detect plant tissues, fungus samples. In this kit, SP can catalyze sucrose to produce glucose 1-phosphate, which is modified to glucose 6-phosphate under the catalysis of glucose phosphomutase, and reduce NADP+ to generate NADPH under the action of glucose 6-phosphate dehydrogenase, resulting in an increase in the light absorption value of 340nm. The SP activity was reflected by the increase rate of 340nm abs

used to detect triglyceridel levels

used to detect cholestrol levels

used to detect Phospholipid levels

The RayBiotech Glutamate Assay Kit provides a simple, reproducible, and sensitive tool of measuring glutamate concentration in plasma, serum, cell lysates, cell culture supernatants, and other biological liquid samples.

The RayBiotech Glutamate Assay Kit provides a simple, reproducible, and sensitive tool of measuring glutamate concentration in plasma, serum, cell lysates, cell culture supernatants, and other biological liquid samples.

The RayBiotech Glutamate Assay Kit provides a simple, reproducible, and sensitive tool of measuring glutamate concentration in plasma, serum, cell lysates, cell culture supernatants, and other biological liquid samples.

The RayBiotech Glycogen Assay Kit provides a simple, reproducible, and sensitive tool of measuring glycogen concentration in cell lysates, urine, saliva, and other biological liquid samples.