Cell Culture devices

Tubing kit to connect ExiGo syringe pump to flow sensor and from flow sensor to input of microfluidic chip. Suitable for low flow rates and general microfluidic applications. Fluid path: • From syringe to flow sensor: Adapter (luer lock ↔︎ 1/32" black connector) to red PEEK tubing (50cm length) to 1/32" black connector . • Flow sensor to chip: 1/32" black connector to red PEEK tubing (50cm length) pushfit to Tygon tubing (30cm length) with stainless steel pin for connection to microfluidic chip. Tubing / Connector / Adapter specifications: • Adapter: luer lock ↔︎ 1/32" black connector • Flow sensor connectors are black nut and ferrule, 0.8mm = 1/32" • Compatible with flow sensors: 1.5μL/min, 7.0μL/min, 80μL/min • Red PEEK tubing: ID: 130μm = 0.13mm; OD: 800μm = 0.8mm = 1/32" • Tygon tubing: ID: 440μm = 0.44mm • Stainless steel pin: 25 gauge thinwall; 17mm length Not suitable for use with whole blood / platelet aggregation / thrombosis assays.

Tubing kit to connect ExiGo syringe pump to flow sensor and from flow sensor to input of microfluidic chip. Suitable for high flow rates, for general microfluidic applications and cell (leukocyte) adhesion assays. Fluid path: • From syringe to flow sensor: Adapter (luer lock ↔︎ 1/4"-UNF female connector) to 1/4" UNF female connector to PTFE tubing (50cm length) to 1/4" UNF female connector. • Flow sensor to chip: 1/4" UNF female connector to ADAPTER to blue luer lock (with needle output) pushfit to Tygon tubing (50cm length) with stainless steel pin for connection to microfluidic chip. Tubing / Connector / Adapter specifications: • Adapter: (luer lock ↔︎ 1/4"-UNF female connector • 1/4"-UNF female connectors • PTFE tubing: ID: 1.0mm (0.04"); OD: 1.6mm (1/16") • Compatible with flow sensors: 1mL/min • ADAPATER (before blue luer lock) • Blue luer lock with 25G needle • Tygon tubing: ID: 440μm = 0.44mm • Stainless steel pin: 25 gauge thinwall; 17mm length Not suitable for use with

Tubing kit to connect ExiGo syringe pump to microfluidic chip via large bore tubing suitable for high flow rates; for general microfluidic applications and thrombosis / platelet aggreation / whole blood studies Fluid path: • From syringe to blue luer lock with 25G needle connector via PVC high pressure tubing (60 cm length). • From blue luer lock to microfluidic chip via Tygon tubing (50cm length) with stainless steel pin for connection to microfluidic chip. Tubing / Connector / Adapter specifications: • PVC high pressure tubing: ID: 1.0mm • Blue luer lock with 25G needle • Tygon tubing: ID: 440μm = 0.44mm • Stainless steel pin: 25 gauge thinwall; 17mm length Not suitable for use with flow sensors.

Tubing kit to connect UniGo pressure pump to microfluidic chip via pressurised 15mL reservoir and flow sensor. Suitable for droplet generation applications. Fluid path: • From pump to 15mL Sample reservoir: Blue pneumatic tubing (30cm length) • From 15mL sample reservoir to eac flow sensor: 1/32" black connector (VICI connector) to blue PEEK tubing (50cm length) to 1/32" black connector (VICI connector). • From 80uL/min flow sensor (oil phase) to Cellix's Dropchip: 1/32" black connector (VICI connector) to blue PEEK tubing (50cm length) - directly into DropChip. • From 7uL/min flow sensor (aqueous phase, sample) to Cellix's Dropchip: 1/32" black connector (VICI connector) to red PEEK tubing (50cm length) - directly into DropChip. Tubing / Connector / Adapter specifications: • Blue Pneumatic Tubing: ID: 2.5mm (0.1”); OD: 4mm (5/32”) • Black connector: nut and ferrule, 0.8mm = 1/32" • Blue PEEK Tubing: ID: 0.25mm (0.01”); OD: 0.8mm (1/32”) • Red PEEK Tubing: ID: 0.13mm (0.005”); OD:

Complete tubing kit for pump including flow sensor tubing kit and culture bottle reservoir.

1 x UniGo pump; no compressor included; 1 x iPad mini with SmartFlo App; 1 x flow sensor (choose from options listed: for flow rates up to 1.5µL/min (FS1.5); 7.0µL/min (FS7.0); 80.0µL/min (FS80.0) or 1mL/min (FS1000)); 1 x tubing kit; power supply and cables.

1 x UniGo pump; no compressor included; 1 x SmartFlo for PC; 1 x flow sensor (choose from options listed: for flow rates up to 1.5µL/min (FS1.5); 7.0µL/min (FS7.0); 80.0µL/min (FS80.0) or 1mL/min (FS1000)); 1 x tubing kit; power supply and cables.

1 x UniGo pump; no compressor included; 1 x flow sensor (choose from options listed: for flow rates up to 1.5µL/min (FS1.5); 7.0µL/min (FS7.0); 80.0µL/min (FS80.0) or 1mL/min (FS1000)); 1 x tubing kit; power supply and cables.

Each biochip contains 4 T-junction shaped capillaries in parallel which can be coated with different adhesion molecules for cell receptor-ligand (i.e. cell-matrix interactions) studies under shear flow with dual-injection (chemotaxis experiments) or for investigation of thrombi formation at the T-channel branch. Each pack contains 10 biochips = 40 assays. Dimensions of microchannels: 400 (W) x 100 (D). Compatible with Mirus Evo Nanopump, Kima pump. Compatible with ExiGo, UniGo, 4U, Mirus and Kima Pumps.